Department of Oncology, Ningbo Mingzhou Hospital, 188 Taian Xi Road, Ningbo, 315899, China.
Mol Cell Biochem. 2013 Oct;382(1-2):137-43. doi: 10.1007/s11010-013-1728-1. Epub 2013 Jun 21.
Resveratrol (RSV), a polyphenol, is known to play an important role in inhibiting proliferation and inducing apoptosis of glioma cells. The aim of this study was to explore the mechanism of RSV on U251 cells apoptosis. RSV showed a dose-dependent decrease in U251 cell viability. It could reduce IκB phosphorylation, nuclear P65 protein levels and NF-κB transcriptional activity, which suggested that signaling pathway are involved in RSV-induced apoptosis. In addition, RSV could inhibit miR-21 expression and down-regulation of miR-21 expression could suppress NF-κB activity. Interestingly, over-expression of miR-21 can reverse the effect of RSV on NF-κB activity and apoptosis in U251 cells. These results suggest that RSV can effectively induce apoptosis of U251 cells and modulation of miR-21 possibly contributes to this antitumor action.
白藜芦醇（RSV）是一种多酚，已知在抑制神经胶质瘤细胞增殖和诱导细胞凋亡方面发挥重要作用。本研究旨在探讨 RSV 诱导 U251 细胞凋亡的机制。RSV 呈剂量依赖性降低 U251 细胞活力。它可以减少 IκB 磷酸化、核 P65 蛋白水平和 NF-κB 转录活性，表明信号通路参与了 RSV 诱导的细胞凋亡。此外，RSV 可以抑制 miR-21 的表达，下调 miR-21 的表达可以抑制 NF-κB 活性。有趣的是，miR-21 的过表达可以逆转 RSV 对 U251 细胞 NF-κB 活性和凋亡的影响。这些结果表明 RSV 可以有效诱导 U251 细胞凋亡，miR-21 的调节可能有助于这种抗肿瘤作用。
