College of Biology and Environmental Sciences, Jishou University, Jishou, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Sep;104(3):331-7. doi: 10.1007/s10482-013-9955-8. Epub 2013 Jun 21.
A novel non-sporulating, non-motile, catalase-positive, oxidase-negative, facultatively anaerobic, Gram-positive coccus, designated strain JSM 078151(T), was isolated from an intertidal sediment sample collected from Naozhou Island in the South China Sea, China. Growth was found to occur in the presence of 0-15 % (w/v) NaCl (optimum 0.5-3 % (w/v) NaCl), at pH 6.5-10.5 (optimum pH 7.0-8.0) and at 5-35 °C (optimum 25-30 °C). The peptidoglycan type was determined to be A3a, containing lysine, glutamic acid and alanine. The major cellular fatty acid identified was anteiso-C15:0 and the predominant menaquinones are MK-7 and MK-8. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unidentified phospholipid. The genomic DNA G+C content of strain JSM 078151(T) was determined to be 55.2 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078151(T) should be assigned to the genus Rothia, and was most closely related to Rothia nasimurium CCUG 35957(T) (98.3 % sequence similarity), followed by Rothia amarae J18(T) (97.5 %) and Rothia terrae L-143(T) (97.3 %). A combination of phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the suggestion that strain JSM 078151(T) represents a novel species of the genus Rothia, for which the name Rothia marina sp. nov. is proposed. The type strain is JSM 078151(T) (= DSM 21080(T) = KCTC 19432(T)).
一株新型非孢子形成、非运动、过氧化氢酶阳性、氧化酶阴性、兼性厌氧、革兰氏阳性球菌，命名为 JSM 078151(T) 菌株，从中国南海南洲岛的潮间带沉积物样本中分离得到。发现该菌在 0-15%（w/v）NaCl（最佳 0.5-3%（w/v）NaCl）、pH 6.5-10.5（最佳 pH 7.0-8.0）和 5-35°C（最佳 25-30°C）的条件下生长。肽聚糖类型被确定为 A3a，含有赖氨酸、谷氨酸和丙氨酸。鉴定出的主要细胞脂肪酸为 anteiso-C15:0，主要的menaquinones 是 MK-7 和 MK-8。极性脂由双磷脂酰甘油、磷脂酰甘油、糖脂和一种未鉴定的磷脂组成。菌株 JSM 078151(T) 的基因组 DNA G+C 含量为 55.2 mol%。基于 16S rRNA 基因序列比较的系统发育分析表明，菌株 JSM 078151(T) 应归属于罗氏菌属，与 Rothia nasimurium CCUG 35957(T)（98.3%序列相似性）最为密切相关，其次是 Rothia amarae J18(T)（97.5%）和 Rothia terrae L-143(T)（97.3%）。系统发育分析、DNA-DNA 同源性值、表型特征和化学生物分类数据的结合表明，菌株 JSM 078151(T) 代表罗氏菌属的一个新种，建议将其命名为 Rothia marina sp. nov.。模式菌株为 JSM 078151(T)（=DSM 21080(T)=KCTC 19432(T)）。
