Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Campus UFSM, Prédio 20, sala 4139, Santa Maria, RS, 97105-900, Brazil.
Mycopathologia. 2013 Aug;176(1-2):165-9. doi: 10.1007/s11046-013-9672-x. Epub 2013 Jun 23.
Here, we evaluated combinations of diphenyl diselenide [(PhSe)2] with fluconazole and amphotericin B in a checkerboard assay against clinical Candida glabrata strains. Minimal inhibitory concentration (geometric mean) ranged from 0.25 to >64 (5.16 μg/mL) for (PhSe)2, 1 to 32 (5.04 μg/mL) for fluconazole and 0.06 to 0.5 (0.18 μg/mL) for amphotericin B. Synergistic (76.66 %) and indifferent (23.34 %) interactions were observed for (PhSe)2 + amphotericin B combination. (PhSe)2 + fluconazole combination demonstrated indifferent (50 %) and antagonistic (40 %) interactions, whereas synergistic interactions were observed in 10 % of the isolates. New experimental in vivo protocols are necessary and will promote a better understanding of the antimicrobial activity of (PhSe)2 against C. glabrata and its use as an adjuvant therapy with antifungal agents.
在这里，我们评估了二苯并二硒醚 [(PhSe)2] 与氟康唑和两性霉素 B 的联合用药，通过棋盘试验对临床光滑念珠菌菌株进行了评估。（PhSe）2 的最小抑菌浓度（几何平均值）范围为 0.25 至 >64（5.16 μg/mL），氟康唑为 1 至 32（5.04 μg/mL），两性霉素 B 为 0.06 至 0.5（0.18 μg/mL）。（PhSe）2 + 两性霉素 B 联合用药观察到协同（76.66%）和无关（23.34%）相互作用。（PhSe）2 + 氟康唑联合用药表现出无关（50%）和拮抗（40%）相互作用，而在 10%的分离物中观察到协同相互作用。新的实验性体内方案是必要的，这将促进更好地了解（PhSe）2 对 C. glabrata 的抗菌活性及其作为抗真菌药物辅助治疗的用途。
