Department of Microbiology and Parasitology, Federal University of Santa Maria (UFSM), Santa Maria, Brazil.
Integrated Regional University of High Uruguay and Missions (URI), Santiago, Brazil.
Mycoses. 2019 May;62(5):428-433. doi: 10.1111/myc.12906. Epub 2019 Mar 13.
This study evaluated the in vitro susceptibility of Trichosporon asahii strains to diphenyl diselenide (DPDS) and ebselen (EBS) alone and in combination with amphotericin B (AMB), fluconazole (FCZ), itraconazole (ITZ) and caspofungin (CAS) using the microdilution method. EBS showed in vitro activity against T asahii strains with minimal inhibitory concentration (MIC) ranged from 0.25 to 8.0 μg/mL. For DPDS, the MIC ranged from 8.0 to 64 μg/mL. The combinations demonstrating the greatest synergism rate against fluconazole-resistant T asahii strains were the following: CAS + DPDS (96.67%), AMB + DPDS (93.33%), FCZ + DPDS (86.67%) and ITZ + DPDS (83.33%). The combinations AMB + DPDS and AMB + EBS exhibited the highest synergism rate against the fluconazole-susceptible (FS) T asahii strains (90%). Antagonism was observed in the following combinations: FCZ + EBS (80%) and FCZ + DPDS (13.33%) against the FS strains, and ITZ + EBS (20%) against the FR strains. Our findings suggest that the antimicrobial activity of DPDS and EBS against T. asahii and its use as an adjuvant therapy with antifungal agents warrant in vivo experimental investigation.
本研究采用微量稀释法评估二苯基二硒醚（DPDS）和艾地苯（EBS）单独及与两性霉素 B（AMB）、氟康唑（FCZ）、伊曲康唑（ITZ）和卡泊芬净（CAS）联合应用时对新生隐球菌的体外药敏活性。EBS 对新生隐球菌的 MIC 范围为 0.25-8.0μg/mL。DPDS 的 MIC 范围为 8.0-64μg/mL。对氟康唑耐药的新生隐球菌菌株显示出最大协同作用的组合如下：CAS+DPDS（96.67%）、AMB+DPDS（93.33%）、FCZ+DPDS（86.67%）和 ITZ+DPDS（83.33%）。AMB+DPDS 和 AMB+EBS 对氟康唑敏感（FS）的新生隐球菌菌株表现出最高的协同作用率（90%）。在以下组合中观察到拮抗作用：FCZ+EBS（80%）和 FCZ+DPDS（13.33%）对 FS 菌株，以及 ITZ+EBS（20%）对 FR 菌株。我们的研究结果表明，DPDS 和 EBS 对新生隐球菌的抗菌活性及其作为抗真菌药物的辅助治疗的应用值得进行体内实验研究。
