Zoucas E, Thörne J, Fäldt R, Ankerst J
Department of Surgery, Lund University Hospital, Sweden.
Crit Care Med. 1990 Aug;18(8):845-7. doi: 10.1097/00003246-199008000-00010.
The effect of granulocytes, stimulated in vivo by standardized soft tissue trauma, on the aggregation of autologous platelets in vitro, was studied in the pig. Platelet aggregation induced by ADP and arachidonic acid was inhibited when platelets harvested before trauma were incubated with granulocytes obtained 5 min after trauma. The granulocytes were separated from the platelets during the incubation by enclosure in dialysis tubes. Platelet inhibition was evident when the cell cohorts were suspended in cell-free medium as well as in autologous plasma extracted before trauma. In addition, incubation of platelets harvested before trauma with plasma obtained after trauma decreased platelet aggregation. In conclusion, granulocytes stimulated in vivo inhibited platelet function in vitro. Platelet inhibitory activity was shown to be related with the granulocytes per se, but also with factors present in plasma after trauma.
在猪身上研究了经标准化软组织创伤在体内刺激的粒细胞对自体血小板体外聚集的影响。当将创伤前采集的血小板与创伤后5分钟获得的粒细胞一起孵育时，由ADP和花生四烯酸诱导的血小板聚集受到抑制。在孵育过程中，通过将粒细胞封闭在透析管中来使其与血小板分离。当细胞群体悬浮于无细胞培养基以及创伤前提取的自体血浆中时，血小板抑制作用明显。此外，将创伤前采集的血小板与创伤后获得的血浆一起孵育会降低血小板聚集。总之，体内受刺激的粒细胞在体外抑制血小板功能。血小板抑制活性显示不仅与粒细胞本身有关，还与创伤后血浆中存在的因子有关。
