Wolanczyk J P, Storey K B, Baust J G
Center for Cryobiological Research, State University of New York, University Center, Binghamton 13901.
Cryobiology. 1990 Jun;27(3):328-35. doi: 10.1016/0011-2240(90)90032-y.
Although the presence of antifreeze and ice nucleating agents in the hemolymph of insects has been well documented, there have been no reports of either of these types of agent in vertebrates. The technique of differential scanning calorimetry was used to examine the blood, serum, and plasma of a freeze-tolerant frog, Rana sylvatica, for the presence of antifreeze protein activity. Results demonstrate the absence of antifreeze protein but the presence of an ice nucleating agent that may serve as a functional component of the overwintering strategy of this species. Ice nucleating activity was detected in samples of cell-free blood, serum, and plasma, suggesting that the agent is a soluble component and possibly plasma protein. To our knowledge, the identification of ice nucleating activity in this freeze-tolerant vertebrate is novel.
尽管昆虫血淋巴中抗冻剂和冰核剂的存在已有充分记载，但脊椎动物中尚未有关于这两种物质的报道。采用差示扫描量热法技术检测了耐冻蛙（林蛙）的血液、血清和血浆中是否存在抗冻蛋白活性。结果表明，抗冻蛋白不存在，但存在一种冰核剂，它可能是该物种越冬策略的一个功能组成部分。在无细胞血液、血清和血浆样本中检测到冰核活性，这表明该物质是一种可溶性成分，可能是血浆蛋白。据我们所知，在这种耐冻脊椎动物中鉴定出冰核活性是前所未有的。
