Chen Shuang-Tao, Zhang Wei-Ping, Liu Chang-An, Wang Jun-Jiang, Song Heng-Yi, Chai Zhi-wen
Zhongguo Gu Shang. 2013 Mar;26(3):223-6.
To discuss the feasibility of vascular bundle implantation combined with allogeneic bone marrow stromal cells (BMSCs) transplantation in treating rabbit femoral head osteonecrosis and bone defect, in order to explore a new method for the treatment of femoral head necrosis.
Thirty-six New Zealand rabbits were randomly divided into three groups,with 12 rabbits in each group. Bilateral femoral heads of the rabbits were studied in the experiment. The models were made by liquid nitrogen frozen, and the femoral heads were drilled to cause bone defect. Group A was the control group,group B was stem cells transplantaion group of allograft marrow stromal,and group C was stem cells transplantation group of allograft marrow stromal combined with vascular bundle implantation. Three rabbits of each group were sacrificed respectively at 2, 4, 8, 12 weeks after operation. All specimens of the femoral heads were sliced for HE staining. Furthermore ,vascular density and the percentage of new bone trabecula of femoral head coronary section in defect area were measured and analyzed statistically.
In group C,new bone trabecula and original micrangium formed at the 2nd week after operation; new bone trabecula was lamellar and interlaced with abundant micrangium at the 8th week;at the 12th week,the broadened,coarsened bone trabecula lined up regularly,and the mature bone trabecula and new marrow were visible. At the 2nd week after operation,there was no statistical significance in the percentage of new bone trabecula of femoral head coronary section in defect area between group B and C. While at 4, 8, 12 week after operation, vascular density and the percentage of new bone trabecula of femoral head coronary section in defect area of group C was higher than that of group B.
Allogeneic bone marrow stromal cells cultured in vivo can form new bone trabecula, and can be applied to allotransplant. Vascular bundle implanted into the bone defect area of femoral head necrosis could improve blood supply, and promote the formation of bone trabecula.
探讨血管束植入联合同种异体骨髓基质细胞（BMSCs）移植治疗兔股骨头坏死及骨缺损的可行性，以探寻治疗股骨头坏死的新方法。
36只新西兰兔随机分为3组，每组12只。实验研究兔双侧股骨头。采用液氮冷冻法制作模型，并在股骨头钻孔造成骨缺损。A组为对照组，B组为同种异体骨髓基质干细胞移植组，C组为同种异体骨髓基质干细胞移植联合血管束植入组。术后2、4、8、12周分别处死每组3只兔子。将所有股骨头标本切片行苏木精-伊红（HE）染色。此外，测量并统计分析股骨头冠状切面缺损区的血管密度及新骨小梁百分比。
C组术后第2周即有新骨小梁及原有微血管形成；第8周新骨小梁呈板层状且相互交织，微血管丰富；第12周骨小梁增宽、变粗，排列规则，可见成熟骨小梁及新骨髓。术后第2周，B组与C组股骨头冠状切面缺损区新骨小梁百分比差异无统计学意义。而术后4、8、12周，C组股骨头冠状切面缺损区的血管密度及新骨小梁百分比均高于B组。
体内培养的同种异体骨髓基质细胞可形成新骨小梁，可应用于异体移植。将血管束植入股骨头坏死骨缺损区可改善血供，促进骨小梁形成。
