Karakus E, Pekyardimchi Sh, Kilich E
Prikl Biokhim Mikrobiol. 2013 Mar-Apr;49(2):209-14. doi: 10.7868/s0555109913020062.
Glucose potentiometric biosensor was prepared by immobilizing glucose oxidase on iodide-selective electrode. The hydrogen peroxide formed after the oxidation of glucose catalysed by glucose oxidase (GOD) was oxidized by sodium molybdate (SMo) at iodide electrode in the presence ofdichlorometane. The glucose concentration was calculated from the decrease of iodide concentration determined by iodide-selective sensor. The sensitivity of glucose biosensor towards iodide ions and glucose was in the concentration ranges of 1.0 x 10(-1) - 1.0 x 10(-6) M and 1.0 x 10(-2) - 1.0 x 10(-4) M, respectively. The characterization of proposed glucose biosensor and glucose assay in human serum were also investigated.
通过将葡萄糖氧化酶固定在碘离子选择性电极上制备了葡萄糖电位生物传感器。在二氯甲烷存在下，葡萄糖氧化酶（GOD）催化葡萄糖氧化后形成的过氧化氢在碘离子电极上被钼酸钠（SMo）氧化。根据碘离子选择性传感器测定的碘离子浓度降低来计算葡萄糖浓度。葡萄糖生物传感器对碘离子和葡萄糖的灵敏度分别在1.0×10⁻¹ - 1.0×10⁻⁶ M和1.0×10⁻² - 1.0×10⁻⁴ M的浓度范围内。还研究了所提出的葡萄糖生物传感器的表征以及人血清中的葡萄糖测定。
