Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, SW 2109, Australia.
J Proteomics. 2013 Aug 26;89:202-14. doi: 10.1016/j.jprot.2013.06.017. Epub 2013 Jun 21.
Echinostomes are cosmopolitan parasites that infect a large number of different warm-blooded hosts, both in nature and in the laboratory. They also constitute an important group of food-borne trematodes of public health importance mainly in Southeast Asia and the Far East. In addition, echinostomes are an ideal model to study several aspects of intestinal helminth biology, since they present a number of advantages. For example, echinostomes are large worms whose life cycle is relatively easy to maintain in the laboratory. Recently, several studies documented their great value in the study of intestinal helminth-vertebrate host relationship. Detailed knowledge of their genome, transcriptome and proteome is likely to have an important impact on the development of control strategies for intestinal helminths. We present the first transcriptome of the adult stage of Echinostoma caproni using 454 sequencing coupled to a semi-automated bioinformatic analyses. 557,236 raw sequence reads were assembled into 28,577 contiguous sequences using iAssembler. 23,296 putative proteins were characterized based on homology, gene ontology and/or biochemical pathways. Comparisons of the transcriptome of E. caproni with those of other trematodes revealed similarities in the transcription pattern of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins like kinases and peptidases were abundant. Of the 3415 predicted excretory/secretory proteins compiled (including non-classical secretory proteins), 180 different proteins were confirmed by proteomic analysis. Potential drug targets were also identified.
In this study the first transcriptome of the adult stage of E. caproni is presented and compared to those of other trematodes revealing similarities in transcription for molecules inferred to have key roles in parasite-host interactions. 3415 predicted excretory/secretory proteins were compiled, being 180 different proteins confirmed by proteomic analysis. The current transcriptome data increased by nine times the number of previous protein identifications. In addition, potential drug targets for this parasite were identified. The present dataset should provide a solid foundation for future fundamental genomic, proteomic, and metabolomic explorations of E. caproni, as well as a basis for applied outcomes, such as the development of novel methods of intervention against this model organism and related parasites.
棘口吸虫是一种世界性的寄生虫，感染了大量不同的温血宿主，包括自然宿主和实验室宿主。它们也是东南亚和远东地区重要的食源性吸虫组群之一，对公共卫生具有重要意义。此外，棘口吸虫是研究肠道寄生虫生物学的理想模型，因为它们具有许多优势。例如，棘口吸虫是大型蠕虫，其生命周期在实验室中相对容易维持。最近，有几项研究记录了它们在研究肠道寄生虫与脊椎动物宿主关系方面的巨大价值。对其基因组、转录组和蛋白质组的详细了解可能会对肠道寄生虫的控制策略的发展产生重要影响。我们使用 454 测序和半自动生物信息学分析，展示了棘口属吸虫 Echinostoma caproni 成虫阶段的第一个转录组。使用 iAssembler 将 557,236 条原始序列组装成 28,577 条连续序列。基于同源性、基因本体和/或生化途径对 23,296 个假定蛋白进行了特征描述。将 E. caproni 的转录组与其他吸虫的转录组进行比较，发现了在推断对寄生虫-宿主相互作用具有关键作用的分子的转录模式上的相似性。酶蛋白如激酶和肽酶很丰富。在编译的 3415 种预测的排泄/分泌蛋白（包括非经典分泌蛋白）中，通过蛋白质组学分析证实了 180 种不同的蛋白。还确定了潜在的药物靶点。
在这项研究中，展示了棘口属吸虫 E. caproni 成虫阶段的第一个转录组，并与其他吸虫的转录组进行了比较，发现了在推断对寄生虫-宿主相互作用具有关键作用的分子的转录模式上的相似性。编译了 3415 种预测的排泄/分泌蛋白，通过蛋白质组学分析证实了 180 种不同的蛋白。与以前的蛋白质鉴定相比，当前的转录组数据增加了 9 倍。此外，还确定了这种寄生虫的潜在药物靶点。本数据集应为未来对 E. caproni 的基础基因组、蛋白质组和代谢组学探索提供坚实的基础，也为应用成果提供了基础，例如开发针对这种模式生物和相关寄生虫的新干预方法。
