Department of Life Science, National Taiwan University, Taipei, Taiwan, ROC.
Biochem Biophys Res Commun. 2013 Jul 26;437(2):232-8. doi: 10.1016/j.bbrc.2013.06.046. Epub 2013 Jun 21.
Membrane type 1 (MT1)-MMP is a member of matrix metalloproteinases (MMPs) that regulates extracellular matrix remodeling. In addition, MT1-MMP also serves as a multi-functional protein. However, the functional role of MT1-MMP in human endothelial cells remains unclear. In this study we use real-time PCR and Western blotting to demonstrate for the first time that MMP-2 expression is regulated by MT1-MMP in human endothelial cells. Moreover, MMP-2 activity is also modulated by MT1-MMP. In addition we found that endothelial cells, ECM adhesion and human endothelial cell tube formation, which are known to be regulated by MMP-2, are blocked by MT1-MMP siRNA. These results suggest that MT1-MMP plays an important role in regulating angiogenesis in human endothelial cells.
膜型 1 基质金属蛋白酶（MT1-MMP）是细胞外基质重塑的基质金属蛋白酶（MMPs）家族的一员。此外，MT1-MMP 也是一种多功能蛋白。然而，MT1-MMP 在人内皮细胞中的功能作用尚不清楚。在这项研究中，我们首次使用实时 PCR 和 Western blot 证明 MMP-2 的表达受人内皮细胞中 MT1-MMP 的调控。此外，MMP-2 的活性也受到 MT1-MMP 的调节。此外，我们发现，已知受 MMP-2 调节的内皮细胞、细胞外基质黏附和人内皮细胞管形成被 MT1-MMP siRNA 阻断。这些结果表明 MT1-MMP 在调节人内皮细胞血管生成中起重要作用。
