Department of Ophthalmology, Saint Roch University Hospital, Nice, France.
Ophthalmic Res. 2013;50(2):83-90. doi: 10.1159/000350550. Epub 2013 Jun 18.
To compare the adherence and structural organization of Staphylococcus epidermidis biofilm on intraocular lenses (IOLs).
IOLs made of 3 different biomaterials [polymethyl methacrylate (PMMA), hydrophilic acrylic or hydrophobic acrylic] were incubated into an S. epidermidis bacterial solution. Scanning electron microscopy was used to count the bound bacteria and to analyze the structural biofilm architecture.
After 4-6 h of incubation, adherence was statistically weakest on the hydrophilic acrylic polymer. On the hydrophobic acrylic material, the bacterial cells tended to cover the substratum in a horizontal spread in a continuous monolayer. On the hydrophilic acrylic material or on the PMMA material bacterial cells tended to form only few, small scattered cell clusters.
The data suggest that the pattern of S. epidermidis adhesion varies with the IOL biomaterial. Hydrophobic IOLs seem to be more permissive to S. epidermidis adhesion.
比较表皮葡萄球菌生物膜在人工晶状体（IOLs）上的黏附及结构组织。
将 3 种不同生物材料[聚甲基丙烯酸甲酯（PMMA）、亲水性丙烯酸或疏水性丙烯酸]制成的 IOL 浸泡在表皮葡萄球菌菌液中。扫描电子显微镜用于计数结合的细菌并分析结构生物膜结构。
孵育 4-6 小时后，亲水性丙烯酸聚合物上的黏附力最弱，具有统计学意义。在疏水性丙烯酸材料上，细菌细胞倾向于在连续单层中水平扩散覆盖基底。在亲水性丙烯酸材料或 PMMA 材料上，细菌细胞往往只形成少量小而分散的细胞簇。
数据表明，表皮葡萄球菌黏附的模式随 IOL 生物材料的不同而变化。疏水性 IOL 似乎更允许表皮葡萄球菌黏附。
