Wang Bailiang, Ye Zi, Tang Yihong, Han Yuemei, Lin Quankui, Liu Huihua, Chen Hao, Nan Kaihui
School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People's Republic of China.
School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou.
Int J Nanomedicine. 2016 Dec 20;12:111-125. doi: 10.2147/IJN.S107472. eCollection 2017.
Infections after surgery or endophthalmitis are potentially blinding complications caused by bacterial adhesion and subsequent biofilm formation on the intraocular lens. Neither single-function anti-adhesion surface nor contacting killing surface can exhibit ideal antibacterial function. In this work, a novel (2-(dimethylamino)-ethyl methacrylate--2-methacryloyloxyethyl phosphorylcholine) (p (DMAEMA--MPC)) brush was synthesized by "grafting from" method through reversible-addition fragmentation chain transfer polymerization. 1-Bromoheptane was used to quaternize the p (DMAEMA--MPC) brush coating and to endow the surface with bactericidal function. The success of the surface functionalization was confirmed by atomic force microscopy, water contact angle, and spectroscopic ellipsometry. The quaternary ammonium salt units were employed as efficient disinfection that can eliminate bacteria through contact killing, whereas the 2-methacryloyloxyethyl phosphorylcholine units were introduced to suppress unwanted nonspecific adsorption. The functionalized poly(dimethyl siloxane) surfaces showed efficiency in reducing bovine serum albumin adsorption and in inhibiting bacteria adhesion and biofilm formation. The copolymer brushes also demonstrated excellent bactericidal function against gram-positive () bacteria measured by bacteria live/dead staining and shake-flask culture methods. The surface biocompatibility was evaluated by morphology and activity measurement with human lens epithelial cells in vitro. The achievement of the p (DMAEMA--MPC) copolymer brush coating with nonfouling, bactericidal, and bacteria corpse release properties can be used to modify intraocular lenses.
手术后感染或眼内炎是由细菌粘附以及随后在人工晶状体上形成生物膜所导致的潜在致盲并发症。单一功能的抗粘附表面或接触杀灭表面均无法展现出理想的抗菌功能。在本研究中，通过可逆加成-断裂链转移聚合反应采用“接枝自”法合成了一种新型的（2-（二甲氨基）乙基甲基丙烯酸酯-2-甲基丙烯酰氧基乙基磷酰胆碱）（p（DMAEMA-MPC））刷。使用1-溴庚烷对p（DMAEMA-MPC）刷涂层进行季铵化处理，赋予其表面杀菌功能。通过原子力显微镜、水接触角和光谱椭偏仪证实了表面功能化的成功。季铵盐单元用作高效消毒剂，可通过接触杀灭消除细菌，而引入2-甲基丙烯酰氧基乙基磷酰胆碱单元以抑制不必要 的非特异性吸附。功能化的聚二甲基硅氧烷表面在减少牛血清白蛋白吸附以及抑制细菌粘附和生物膜形成方面表现出有效性。通过细菌活/死染色和摇瓶培养法测定，共聚物刷对革兰氏阳性（）细菌也表现出优异的杀菌功能。通过体外对人晶状体上皮细胞的形态学和活性测量评估了表面生物相容性。具有抗污、杀菌和细菌尸体释放特性的p（DMAEMA-MPC）共聚物刷涂层的成果可用于修饰人工晶状体。
