Department of Chemistry, Faculty of Science, Hacettepe University, Beytepe-Ankara 06800, Turkey.
Sensors (Basel). 2013 Jun 24;13(7):8079-94. doi: 10.3390/s130708079.
Herein, we report a simple and inexpensive way for the fabrication of an ultramicroelectrode and present its characterization by electrochemical techniques. The fabrication of polyaniline UME involves only two steps: modification of a gold (Au) electrode by self assembled monolayers (SAM) and then electrodeposition of polyaniline film on this thiol-coated Au electrode by using cyclic voltammetry and constant potential electrolysis methods. Two types of self-assembled monolayers (4-mercapto-1-butanol, MB, and 11-mercaptoundecanoic acid, MUA) were used, respectively, to see the effect of chain length on microelectrode formation. Microelectrode fabrication and utility of the surface was investigated by cyclic voltammetric measurements in a redox probe. The thus prepared polyaniline microelectrode was then used for DNA immobilization. Discrimination between double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) was obtained with enhanced electrochemical signals compared to a polyaniline-coated Au electrode. Different modifications on the electrode surfaces were examined using scanning electron microscopy (SEM).
本文报道了一种简单且廉价的制备超微电极的方法，并通过电化学技术对其进行了表征。聚 苯胺 UME 的制备仅包括两个步骤：通过自组装单分子层（SAM）对金（Au）电极进行修饰，然后通过循环伏安法和恒电位电解法在该巯基涂覆的 Au 电极上电沉积聚苯胺膜。分别使用两种自组装单分子层（4-巯基-1-丁醇（MB）和 11-巯基十一酸（MUA）），以观察链长对微电极形成的影响。通过在氧化还原探针中进行循环伏安测量来研究微电极的制备和表面的用途。然后，将制备的聚苯胺微电极用于 DNA 固定化。与涂覆有聚苯胺的 Au 电极相比，通过增强的电化学信号获得了双链 DNA（dsDNA）和单链 DNA（ssDNA）之间的区分。使用扫描电子显微镜（SEM）检查了电极表面的不同修饰。
