Wang Jin, Xu Zhen, Peng Shu-Chuan, Xia Ming-Shan, Yue Zheng-Bo, Chen Tian-Hn
School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China.
Huan Jing Ke Xue. 2013 Apr;34(4):1510-6.
In this study, two halophilic bacteria were isolated from activated sludge in the epoxy wastewater treatment system. The strains were identified, and the growth and degradation characteristics were investigated. Strain J1 and J2 was identified respectively by morphological observation and 16S rDNA sequence alignment analysis. It was found that both strains belong to the Bacillus genus (Bacillus sp.) and branch Bacillus (Virgibacillus sp.). The optimized growth condition of strain J1 and J2 in the high salt CM culture medium was as follows: solution temperature 30 degrees C, pH 7.0 and 5-50 g x L(-1) of NaCl. Furthermore, the best degradation condition of the organic epoxy wastewater was: temperature 30 degrees C, pH 7.0 and NaCl concentration 30 g x L(-1). When the volume ratio of bacterial suspension mixture of J1 and J2 was 2:1 and the inoculum size of the composite strains was 10%, the highest COD removal efficiency was achieved in the epoxy wastewater treatment.
在本研究中,从环氧废水处理系统的活性污泥中分离出两株嗜盐细菌。对这些菌株进行了鉴定,并研究了其生长和降解特性。通过形态学观察和16S rDNA序列比对分析分别对菌株J1和J2进行了鉴定。结果发现,这两株菌株均属于芽孢杆菌属(芽孢杆菌种)和分支芽孢杆菌属(类芽孢杆菌种)。菌株J1和J2在高盐CM培养基中的优化生长条件如下:溶液温度30℃,pH值7.0,NaCl浓度为5 - 50 g·L⁻¹。此外,有机环氧废水的最佳降解条件为:温度30℃,pH值7.0,NaCl浓度30 g·L⁻¹。当J1和J2的细菌悬浮液混合体积比为2:1且复合菌株接种量为10%时,在环氧废水处理中COD去除效率最高。