Karn Santosh Kr, Reddy M Sudhakara
Department of Biotechnology, Thapar University, Patiala, 147004, Punjab, India.
J Gen Appl Microbiol. 2012;58(6):413-20. doi: 10.2323/jgam.58.413.
Bacterial strains capable of degrading trichlorophenol (2,4,6-TCP) were isolated from the secondary sludge of a pulp and paper mill and were characterized. These isolates were identified as Planococcus rifietoensis (CL4) and Bacillus pumilus (CL5), based on their 16S rRNA sequence analysis. These isolates were able to grow and utilize 2,4,6-TCP as their source of carbon as well as energy. HPLC analysis and stoichometric release of chloride in the medium confirmed the degradation ability of these isolates. Removal efficiency of 2,4,6-TCP by these isolates was discovered to be high. They were able to remove 90% of 2,4,6-TCP when grown at a concentration of 600 mg L(-1). Inoculation of these bacteria completely removed 2,4,6-TCP within 2 weeks from the sludge of the pulp and paper mill when supplemented at the rate of 100 mg L(-1). Absorbable Organic Halogen (AOX) and Extractable Organic Halogen (EOX) were significantly reduced by 63% and 70% respectively from the sludge due to inoculation of these bacteria. These isolates have high potential to remove 2,4,6-TCP and may be used for removal of 2,4,6-TCP from pulp paper mill waste.
从一家造纸厂的二次污泥中分离出了能够降解三氯苯酚（2,4,6 - TCP）的细菌菌株，并对其进行了表征。基于16S rRNA序列分析，这些分离株被鉴定为里菲托平原球菌（CL4）和短小芽孢杆菌（CL5）。这些分离株能够生长并利用2,4,6 - TCP作为碳源和能源。高效液相色谱分析以及培养基中氯离子的化学计量释放证实了这些分离株的降解能力。发现这些分离株对2,4,6 - TCP的去除效率很高。当在600 mg L⁻¹的浓度下生长时，它们能够去除90%的2,4,6 - TCP。以100 mg L⁻¹的速率添加这些细菌时，在两周内可将造纸厂污泥中的2,4,6 - TCP完全去除。由于接种了这些细菌，污泥中的可吸收有机卤素（AOX）和可提取有机卤素（EOX）分别显著降低了63%和70%。这些分离株具有很高的去除2,4,6 - TCP的潜力，可用于去除造纸厂废水中的2,4,6 - TCP。
