Hironaka N, Miyata H, Takada K, Ando K
Department of Psychopharmacology, Preclinical Research Laboratories, Kawasaki.
Nihon Yakurigaku Zasshi. 1990 Jun;95(6):309-18. doi: 10.1254/fpj.95.6_309.
Delayed discrimination experiments were conducted to examine drug effects on memory in rats. Three kinds of experimental situations: a Y-maze, an operant chamber with two retractable levers, and an operant chamber with two fixed levers were used. A discriminative stimulus light either on the left or the right side of a stimulus panel was presented for a brief period in each situation. After a certain delay time following extinguishment of the light, a choice response to the previously lighted side was termed as a correct choice. Scopolamine at 0.015-0.06 mg/kg, s.c., decreased the correct choice ratio in trials with a delay time of 0 sec in the Y-maze situation and in trials with longer delay times in the operant chamber situations. Nicotine at 0.06-1 mg/kg, s.c., decreased the correct choice ratio in trials with a delay time of 0-4 sec in the Y-maze situation, a delay time of 4 sec in the retractable-lever operant chamber situation, and a delay time of 0.1 sec in the fixed-lever operant chamber situation. Using this delayed discrimination procedure, drug effects on the relationship between delay time and correct choice ratio were observed. From these results, the present procedure was found to be useful for the evaluation of drug effects on memory in rats.
进行延迟辨别实验以研究药物对大鼠记忆的影响。使用了三种实验情境：一个Y型迷宫、一个带有两个可伸缩杠杆的操作箱以及一个带有两个固定杠杆的操作箱。在每种情境中，刺激面板左侧或右侧会短暂呈现一个辨别性刺激光。在光熄灭后的一段延迟时间后，对先前照亮一侧的选择反应被称为正确选择。皮下注射0.015 - 0.06 mg/kg的东莨菪碱，在Y型迷宫情境中延迟时间为0秒的试验以及在操作箱情境中延迟时间较长的试验中，降低了正确选择比例。皮下注射0.06 - 1 mg/kg的尼古丁，在Y型迷宫情境中延迟时间为0 - 4秒的试验、在可伸缩杠杆操作箱情境中延迟时间为4秒的试验以及在固定杠杆操作箱情境中延迟时间为0.1秒的试验中，降低了正确选择比例。使用这种延迟辨别程序，观察到了药物对延迟时间与正确选择比例之间关系的影响。从这些结果来看，发现本程序对于评估药物对大鼠记忆的影响是有用的。
