Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-853, Republic of Korea.
J Microbiol Biotechnol. 2013 Oct 28;23(10):1381-5. doi: 10.4014/jmb.1306.06013.
The centipede Scolopendra subpinipes mutilans is a medicinally important arthropod species. However, its transcriptome is not currently available and transcriptome analysis would be useful in providing insight into a molecular level approach. Hence, we performed de novo RNA sequencing of S. subpinipes mutilans using next-generation sequencing. We generated a novel peptide (scolopendrasin II) based on a SVM algorithm, and biochemically evaluated the in vitro antimicrobial activity of scolopendrasin II against various microbes. Scolopendrasin II showed antibacterial activities against gram-positive and -negative bacterial strains, including the yeast Candida albicans and antibiotic-resistant gram-negative bacteria, as determined by a radial diffusion assay and colony count assay without hemolytic activity. In addition, we confirmed that scolopendrasin II bound to the surface of bacteria through a specific interaction with lipoteichoic acid and a lipopolysaccharide, which was one of the bacterial cell-wall components. In conclusion, our results suggest that scolopendrasin II may be useful for developing peptide antibiotics.
少棘蜈蚣是一种具有重要药用价值的节肢动物。然而，目前还没有关于它的转录组信息，因此对其进行转录组分析有助于深入了解分子水平的机制。因此，我们使用下一代测序技术对少棘蜈蚣进行了 de novo RNA 测序。我们基于 SVM 算法生成了一种新型肽（蜈蚣素 II），并从生化角度评估了蜈蚣素 II 对各种微生物的体外抗菌活性。通过放射状扩散试验和菌落计数试验，蜈蚣素 II 显示出对革兰氏阳性和革兰氏阴性菌、包括酵母白色念珠菌和耐抗生素的革兰氏阴性菌的抗菌活性，而没有溶血活性。此外，我们通过蜈蚣素 II 与细菌细胞壁成分之一的脂磷壁酸和脂多糖的特异性相互作用证实了其与细菌表面的结合。总之，我们的研究结果表明，蜈蚣素 II 可能有助于开发新型肽类抗生素。
