Department of Biological Sciences, Sunway University, Bandar Sunway, Malaysia.
College of Arts and Sciences, American University of Sharjah, Sharjah, UAE.
Folia Microbiol (Praha). 2021 Apr;66(2):285-291. doi: 10.1007/s12223-021-00860-6. Epub 2021 Mar 11.
The evolution of multiple-drug resistant bacteria is contributing to the global antimicrobial crisis, hence driving us to search for novel antimicrobial(s). Among animals, invertebrates represent up to 80% of all known species suggesting their wide distribution. Despite their ubiquitous and plentiful nature, they have been largely unexplored as potential source of antibacterials. In this study, we selected a broad range of invertebrates from terrestrial and marine environments and tested their lysates for antibacterial activity against methicillin-resistant Staphylococcus aereus (MRSA) and neuropathogenic Escherichia coli K1. Cockroaches, centipedes, tarantulas, prawns, lobster, and mud crabs showed antibacterial activity with selected lysates exhibiting more than 90% bactericidal effects. The red-headed centipede's hemolymph showed 90% and 50% bacteriostatic activity against MRSA and E. coli K1, respectively. Tarantula's body extracts exhibited antibacterial activity against MRSA and E. coli K1. Gut extracts of tiger prawn exhibited more than 90% bacteriostatic activity against both bacteria. The selected lobster and mud crab extract exhibited up to 90% growth inhibitory activity against MRSA. Overall, these results showed that selected invertebrates are an untapped source of broad-spectrum antibacterial activity and suggest the presence of biologically active molecules.
多药耐药菌的进化导致了全球抗菌药物危机，因此我们正在寻找新的抗菌药物。在动物中，无脊椎动物占所有已知物种的 80%，这表明它们的分布广泛。尽管它们无处不在且数量丰富，但作为潜在的抗菌药物来源，它们在很大程度上尚未被探索。在这项研究中，我们从陆地和海洋环境中选择了广泛的无脊椎动物，并测试了它们的裂解物对耐甲氧西林金黄色葡萄球菌（MRSA）和神经病原性大肠杆菌 K1 的抗菌活性。蟑螂、蜈蚣、狼蛛、对虾、龙虾和泥蟹的裂解物显示出抗菌活性，其中一些裂解物的杀菌效果超过 90%。红头蜈蚣的血淋巴对 MRSA 和大肠杆菌 K1 的抑菌活性分别为 90%和 50%。狼蛛的身体提取物对 MRSA 和大肠杆菌 K1 均具有抗菌活性。虎虾的肠道提取物对两种细菌均具有超过 90%的抑菌活性。所选龙虾和泥蟹提取物对 MRSA 的生长抑制活性高达 90%。总的来说，这些结果表明，所选无脊椎动物是广谱抗菌活性的未开发来源，并表明存在具有生物活性的分子。
