Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
J Biomed Nanotechnol. 2013 May;9(5):825-32. doi: 10.1166/jbn.2013.1579.
Quantification of the blood glucose concentration in the whole blood was not easy to achieve because the detection process was affected by many factors, such as glucose metabolism and biofouling. In this paper, we established an amperometric glucose biosensor applied in whole blood directly, which was based on the direct electron transfer of glucose oxidase (GOx) entrapped onto the Au-F127 nanospheres. Here, the Au-F127 nanospheres could provide a blood compatible surface with antifouling property for determination of glucose in whole blood. The cyclic voltammetric results indicated that GOx immobilized on the Au-F127 nanospheres exhibited direct electron transfer reaction, and the cyclic voltammogram (CV) displayed a pair of well-defined and nearly symmetric redox peaks with a formal potential of 93 mV. The biosensor had good electrocatalytic activity toward glucose with a low detection limit 3.15 pM. The glucose biosensor did not respond to ascorbic acid (AA) and uric acid (UA) at their high concentration encountered in blood. In this method, the biosensor was used for quantification of the concentration of glucose in whole blood samples. The data obtained from the biosensor showed good agreement with those from a biochemical analyzer in hospital.
由于血糖检测过程受到葡萄糖代谢和生物污垢等多种因素的影响，因此直接在全血中定量检测血糖浓度并不容易。本文建立了一种直接应用于全血的安培型葡萄糖生物传感器，该传感器基于葡萄糖氧化酶（GOx）固定在 Au-F127 纳米球上的直接电子转移。在这里，Au-F127 纳米球为全血中的葡萄糖测定提供了具有抗污性能的血液相容性表面。循环伏安法结果表明，固定在 Au-F127 纳米球上的 GOx 表现出直接电子转移反应，循环伏安图（CV）显示出一对具有良好定义且近乎对称的氧化还原峰，其形式电位为 93 mV。该生物传感器对葡萄糖具有良好的电催化活性，检测限低至 3.15 pM。该葡萄糖生物传感器对血液中遇到的高浓度抗坏血酸（AA）和尿酸（UA）没有响应。在该方法中，生物传感器用于定量检测全血样品中的葡萄糖浓度。生物传感器获得的数据与医院生化分析仪获得的数据吻合良好。
