Sun Chong, Niu Yanlian, Yang Xujie, Liu Min, Yang Xiaodi, Huang Xiaohua, Zhao Wenbo
J Nanosci Nanotechnol. 2015 Jan;15(1):105-11. doi: 10.1166/jnn.2015.8771.
In this paper, the novel poly(lactic-co-glycolic acid)-F127 nanospheres (PLGA-F127 NSs) were synthesized and used to establish an amperometric glucose biosensor that can be applied in whole blood directly. This property of glucose biosensor was based on the antibiofouling property of PLGA-F127 NSs. More details of preparing PLGA-F127 NSs and immobilizing glucose oxidase (GOx) on (PLGA-F127)/glass carbon electrode (GCE) were presented. Then, the electrochemical behaviors of the biosensor in whole blood were studied. The cyclic voltammetric results indicated that GOx immobilized on PLGA-F127 NSs exhibited direct electron transfer reaction, which led to stable amperometric biosensing for glucose with a detection limit of 5.57 x 10(-6) M (S/N = 3). The glucose biosensor did not respond to ascorbic acid (AA) and uric.acid (UA) at their concentration normally encountered in blood. The development of materials science will bring significant input to high-performance biosensors relevant to diagnostics and therapy of interest for human health.
本文合成了新型聚乳酸-乙醇酸共聚物-F127纳米球（PLGA-F127 NSs），并用于构建可直接应用于全血的安培型葡萄糖生物传感器。该葡萄糖生物传感器的这一特性基于PLGA-F127 NSs的抗生物污染特性。文中详细介绍了PLGA-F127 NSs的制备以及葡萄糖氧化酶（GOx）在（PLGA-F127）/玻碳电极（GCE）上的固定化过程。然后，研究了该生物传感器在全血中的电化学行为。循环伏安法结果表明，固定在PLGA-F127 NSs上的GOx表现出直接电子转移反应，从而实现了对葡萄糖的稳定安培生物传感，检测限为5.57×10⁻⁶ M（S/N = 3）。该葡萄糖生物传感器对血液中常见浓度的抗坏血酸（AA）和尿酸（UA）无响应。材料科学的发展将为与人类健康诊断和治疗相关的高性能生物传感器带来重要贡献。
