Ujhelyi Z, Róka E, Fenyvesi F, Fehér P, Váradi J, Réti-Nagy K, Vecsernyés M, Bácskay I
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Hungary.
Pharmazie. 2013 May;68(5):383-4.
The hemolytic activity and the cytotoxicity of PEG-based solubilizing agents on Caco-2 monolayer were investigated. In vitro tests can predict the irritancy potential and the delayed toxicity of the surfactants. There were significant concentration dependent differences between the result of the MTT (3-(4,5-dimethylthiazol-2-yl))-2,5-diphenyltetrazolium bromide) test and the data of the hemolytic activity test. Our investigations show that safer and more applicable tensides can be selected in order to form a more biocompatible medicament.
研究了基于聚乙二醇（PEG）的增溶剂对Caco-2单层细胞的溶血活性和细胞毒性。体外试验可以预测表面活性剂的刺激潜力和延迟毒性。MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐）试验结果与溶血活性试验数据之间存在显著的浓度依赖性差异。我们的研究表明，可以选择更安全、更适用的表面活性剂，以形成生物相容性更好的药物。
