Leakey T E
Joint Academic Department of Child Health, Queen Elizabeth Hospital for Children, London, U.K.
J Chromatogr. 1990 May 16;507:199-220. doi: 10.1016/s0021-9673(01)84196-0.
A method has been developed for the simultaneous determination of methylxanthines and many of their metabolites in plasma. Specially developed extraction columns (Celute-MX) were used and the extracts were separated on a 25-cm ODS column (particle size, 3 microns) at 50 degrees C with a mobile phase gradient. The compounds were detected with a diode array detector at two analytical wavelengths. The value of drug metabolite ratios has been explored and elevated ratios have been found when metabolic clearance was impaired by disease or co-administered drugs. Large concentrations of pharmacologically active metabolites have been found in renal failure. The assay has proved to be reliable and valuable in the elucidation of therapeutic and metabolic problems involving the methylxanthines.
已开发出一种同时测定血浆中甲基黄嘌呤及其多种代谢物的方法。使用了专门开发的萃取柱（Celute-MX），提取物在25厘米的ODS柱（粒径3微米）上于50℃以流动相梯度进行分离。用二极管阵列检测器在两个分析波长下检测这些化合物。已对药物代谢物比率的值进行了探究，并且发现当代谢清除因疾病或联合用药而受损时，比率会升高。在肾衰竭患者中发现了大量具有药理活性的代谢物。该测定法已证明在阐明涉及甲基黄嘌呤的治疗和代谢问题方面是可靠且有价值的。
