Wei Xiao-Qiang, Ma Hui-Qing, Liu Ai-Hong, Zhang You-Zhong
Department of Gynecology and Obstetrics, Qilu Hospital, Shandong Univeristy , Jinan, China.
Asian Pac J Cancer Prev. 2013;14(5):3023-8. doi: 10.7314/apjcp.2013.14.5.3023.
Photodynamic therapy (PDT ) is a promising modality for the treatment of various tumors. In order to assist in optimizing treatment, we applied 5-ALA/PDT in combination with low-dose cisplatin to evaluate cytotoxicity in Hela cells.
Antiproliferative effects of 5-ALA/PDT and cisplatin, alone and in combination, were assessed using MTT assay. To examine levels of apoptosis, Hela cells treated with 5-ALA/PDT, and combination treatment were assessed with Annexin-V/PI by flow cytometry. To investigate the molecular mechanisms underlying alterations in cell proliferation and apoptosis, Western blot analysis was conducted to determine the expression of p53, p21, Bax and Bcl-2 proteins.
MTT assays indicated that combination treatment obviously decreased the viability of Hela cells compared to individual drug treatment. In addition, it was confirmed that exposure of Hela cells to 5-ALA/PDT in combination with low-dose cisplatin resulted in more apoptosis in vitro. Synergistic anticancer activity was related to upregulation p53 expression and alteration in expression of p21, Bcl-2 and Bax.
Our findings suggest that administration of 5-ALA/PDT in combination with the low-dose cisplatin may be an effective and feasible therapy for cervical cancer.
光动力疗法（PDT）是一种很有前景的治疗多种肿瘤的方法。为了辅助优化治疗，我们将5-氨基酮戊酸/光动力疗法（5-ALA/PDT）与低剂量顺铂联合应用，以评估对人宫颈癌HeLa细胞的细胞毒性。
采用MTT法评估5-ALA/PDT和顺铂单独及联合应用的抗增殖作用。为检测凋亡水平，通过流式细胞术用膜联蛋白V/碘化丙啶（Annexin-V/PI）对经5-ALA/PDT处理及联合处理的HeLa细胞进行评估。为研究细胞增殖和凋亡改变的分子机制，进行蛋白质免疫印迹分析以确定p53、p21、Bax和Bcl-2蛋白的表达。
MTT分析表明，与单独药物治疗相比，联合治疗明显降低了HeLa细胞的活力。此外，证实HeLa细胞暴露于5-ALA/PDT联合低剂量顺铂会在体外导致更多细胞凋亡。协同抗癌活性与p53表达上调以及p21、Bcl-2和Bax表达改变有关。
我们的研究结果表明，5-ALA/PDT联合低剂量顺铂给药可能是一种治疗宫颈癌的有效且可行的疗法。
