He Gui-Fang, Bian Mei-Lu, Zhao Ya-Wei, Xiang Qing, Li Hong-Yan, Xiao Cheng
Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 100029, PR China.
Oncol Rep. 2009 Apr;21(4):861-8. doi: 10.3892/or_00000295.
5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) is a new ablation treatment for tumors, while its function mechanism in cervical cancer has not been elucidated. In this study, we investigated the effects of ALA-PDT on the cervical cancer cell line Me180, to search for optimal parameters of PDT. ALA-PDT on proliferation of Me180 was examined by MTT assay to find the optimal function parameters of ALA-PDT. Apoptosis was observed by using AnnexinV-FITC/PI double staining, Hoechst 33342 staining and May-Grünwald-Farbstoff Giemsa staining. Furthermore, we established a tumor model and 6 mice of each group underwent measurement of the tumor size on days 3, 7, 14, and 21 after treatment. The mRNA expression of survivin, bcl-2, p53, bax and bad in Me180 cells were detected by real-time fluorescence reverse transcription-polymerase chain reaction (RT-PCR) in vitro and in vivo. Finally, we compared the effects between topical and intravenous administration. Based on the above studies, we found ALA-PDT induced apoptosis and G0-G1 phase arrest of Me180 cells. The tumor volume of the topical administration and PDT group was the smallest at 7-14 days post-PDT. H&E staining showed remarkable subcutaneous necrosis in the PDT groups. The mRNA expression of survivin and bcl-2 in Me180 cells were suppressed post-PDT. Topical administration of PDT is recommended in treating cervical cancer so as to minimize the side-effects and inconvenience of phototoxic reaction brought by PDT. Our data may contribute to making the mechanism of PDT on cervical cancer clearer and give some useful suggestions for clinical application.
5-氨基酮戊酸介导的光动力疗法（ALA-PDT）是一种新型肿瘤消融治疗方法，但其在宫颈癌中的作用机制尚未阐明。本研究中，我们研究了ALA-PDT对宫颈癌细胞系Me180的影响，以寻找光动力疗法的最佳参数。通过MTT法检测ALA-PDT对Me180增殖的影响，以确定ALA-PDT的最佳作用参数。采用AnnexinV-FITC/PI双染、Hoechst 33342染色和May-Grünwald-Farbstoff吉姆萨染色观察细胞凋亡。此外，我们建立了肿瘤模型，每组6只小鼠在治疗后第3、7、14和21天测量肿瘤大小。通过实时荧光逆转录-聚合酶链反应（RT-PCR）在体外和体内检测Me180细胞中survivin、bcl-2、p53、bax和bad的mRNA表达。最后，我们比较了局部给药和静脉给药的效果。基于上述研究，我们发现ALA-PDT诱导Me180细胞凋亡和G0-G1期阻滞。光动力疗法局部给药组在光动力治疗后7-14天肿瘤体积最小。苏木精-伊红染色显示光动力治疗组皮下有明显坏死。光动力治疗后，Me180细胞中survivin和bcl-2的mRNA表达受到抑制。建议采用光动力疗法局部给药治疗宫颈癌，以尽量减少光动力疗法带来的光毒性反应的副作用和不便。我们的数据可能有助于更清楚地了解光动力疗法治疗宫颈癌的机制，并为临床应用提供一些有用的建议。
