Sarkar Anupam, Bhagat Jacky, Ingole Baban S, Rao Durga P, Markad Vijaykumar L
Chemical Oceanographic Division, CSIR-National Institute of Oceanography Dona Paula, Goa, 403004, India.
Environ Toxicol. 2015 Feb;30(2):177-87. doi: 10.1002/tox.21883. Epub 2013 Jun 27.
This paper presents an evaluation of the genotoxic effects of cadmium chloride (CdCl2 ) on marine gastropod, Nerita chamaeleon following the technique of comet assay and the DNA alkaline unwinding assay (DAUA). In this study, the extent of DNA damage in gill cells of N. chamaeleon was measured after in vivo exposure to four different concentrations (10, 25, 50, and 75 µg/L) of CdCl2 . In vitro exposure of hydrogen peroxide (H2 O2 ; 1, 10, 25, and 50 µM) of the gill cells showed a significant increase in the percentage tail DNA, Olive tail moment, and tail length (TL). Significant changes in percentage tail DNA by CdCl2 exposure were observed in all exposed groups of snails with respect to those in control. Exposure to 75 µg/L of CdCl2 produced significant decrease in DNA integrity as measured by DAUA at all duration with respect to control. In vivo exposure to different concentrations of CdCl2 (10, 25, 50, and 75 µg/L) to N. chamaeleon showed considerable increase in DNA damage as observed by both alkaline comet assay and the DAUA. The extent of DNA damage in marine gastropods determined by the application of alkaline comet assay and DAUA clearly indicated the genotoxic responses of marine gastropod, N. chamaeleon to a wide range of cadmium concentration in the marine environment.
本文采用彗星试验技术和DNA碱性解旋试验（DAUA），评估了氯化镉（CdCl2）对海洋腹足纲动物变色蜒螺的遗传毒性效应。在本研究中，将变色蜒螺体内暴露于四种不同浓度（10、25、50和75μg/L）的CdCl2后，测定其鳃细胞中的DNA损伤程度。鳃细胞体外暴露于过氧化氢（H2O2；1、10、25和50μM）后，尾DNA百分比、橄榄尾矩和尾长（TL）显著增加。与对照组相比，在所有暴露的蜗牛组中，CdCl2暴露均导致尾DNA百分比发生显著变化。与对照组相比，暴露于75μg/L的CdCl2在所有时间段均导致DNA完整性显著下降，这通过DAUA测定。对变色蜒螺体内暴露于不同浓度的CdCl2（10、25、50和75μg/L）后，碱性彗星试验和DAUA均显示DNA损伤显著增加。通过碱性彗星试验和DAUA测定的海洋腹足纲动物的DNA损伤程度清楚地表明了海洋腹足纲动物变色蜒螺对海洋环境中广泛镉浓度的遗传毒性反应。
