Bhagat J, Ingole B S
Biological Oceanographic Division, CSIR-National Institute of Oceanography, Dona Paula, Goa, 403004, India,
Environ Sci Pollut Res Int. 2015 Jul;22(14):10758-68. doi: 10.1007/s11356-015-4263-7. Epub 2015 Mar 12.
In vivo and in vitro exposures were used to investigate the genotoxicity of mercuric chloride (HgCl2) to the marine snail, Planaxis sulcatus. The comet assay protocol was validated on gill cells exposed in vitro to hydrogen peroxide (H2O2, 0-50 μM). Snails were exposed in vivo for 96 h to HgCl2 (10, 20, 50, and 100 μg/l). Our results showed significant concentration-dependent increase in the tail DNA (TDNA) and olive tail moment (OTM) in exposed snails for all doses compared with controls. In vitro exposure to HgCl2 (10-100 μg/l) resulted in significantly higher values for TDNA at all concentrations. Our results showed that DNA damage increased in the gill cell with increasing exposure time. This study demonstrates the usefulness of comet assay for detection of DNA damage after exposure to HgCl2 and the sensitivity of marine snail P. sulcatus as a good candidate species for metal pollution.
采用体内和体外暴露实验来研究氯化汞（HgCl2）对海蜗牛（沟蜷螺，Planaxis sulcatus）的遗传毒性。彗星试验方案在体外暴露于过氧化氢（H2O2，0 - 50 μM）的鳃细胞上得到验证。蜗牛在体内暴露于HgCl2（10、20、50和100 μg/l）96小时。我们的结果显示，与对照组相比，所有剂量的暴露蜗牛的尾部DNA（TDNA）和橄榄尾矩（OTM）均呈现出显著的浓度依赖性增加。体外暴露于HgCl2（10 - 100 μg/l）导致所有浓度下的TDNA值显著更高。我们的结果表明，鳃细胞中的DNA损伤随着暴露时间的增加而增加。本研究证明了彗星试验在检测暴露于HgCl2后的DNA损伤方面的有效性，以及海蜗牛沟蜷螺作为金属污染良好候选物种的敏感性。
