维生素D3代谢产物对培养的大鼠垂体细胞促甲状腺激素分泌的影响。

Effects of vitamin D3 metabolites on thyrotropin secretion from rat pituitary cells in culture.

作者信息

Törnquist K, Forss L, Lamberg-Allardt C

机构信息

Endocrine Research Laboratory, University of Helsinki, Minerva Foundation Institute for Medical Research, Helsinki, Finland.

出版信息

J Endocrinol Invest. 1990 May;13(5):391-5. doi: 10.1007/BF03350686.

DOI:10.1007/BF03350686

PMID:2380504

Abstract

The effects of several vitamin D3 metabolites on TSH secretion were investigated using rat pituitary cells in culture. After a 24-h incubation in Dulbecco's medium, only 25,26-dihydroxyvitamin D3 [25,26(OH)2D3] at 10(-6) M increased the basal TSH secretion (36% above control). The TRH-induced TSH secretion increased dose-dependently with increasing concentrations of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] showing a significant increase at 10(-8) M (41% above control). At 10(-5) M 25,26(OH)2D3 the TRH-stimulated TSH secretion was also increased (63% above control). In medium containing charcoal-treated serum, 1, 24,25-trihydroxyvitamin D3 [1,24,25(OH)3D3] decreased the TRH-induced TSH secretion dose dependently (34% below control at 10(-8) M), while the effects of both 1,25(OH)2D3 and 25,26(OH)2D3 were blunted. Our results indicate that several vitamin D3 metabolites may affect pituitary TSH secretion.

摘要

利用培养的大鼠垂体细胞研究了几种维生素D3代谢产物对促甲状腺激素（TSH）分泌的影响。在杜尔贝科培养基中孵育24小时后，仅10^(-6) M的25,26 - 二羟基维生素D3 [25,26(OH)2D3]可增加基础TSH分泌（比对照高36%）。促甲状腺激素释放激素（TRH）诱导的TSH分泌随1,25 - 二羟基维生素D3 [1,25(OH)2D3]浓度增加呈剂量依赖性增加，在10^(-8) M时显著增加（比对照高41%）。在10^(-5) M的25,26(OH)2D3时，TRH刺激的TSH分泌也增加（比对照高63%）。在含有经活性炭处理血清的培养基中，1,24,25 - 三羟基维生素D3 [1,24,25(OH)3D3]剂量依赖性地降低TRH诱导的TSH分泌（在10^(-8) M时比对照低34%），而1,25(OH)2D3和25,26(OH)2D3的作用均减弱。我们的结果表明，几种维生素D3代谢产物可能影响垂体TSH分泌。