Konohana A, Konohana I, Schroeder W T, O'Brien W R, Amagai M, Greer J, Shimizu N, Gammon W R, Siciliano M J, Duvic M
Department of Dermatology and Internal Medicine, University of Texas Medical School, Houston 77030.
J Invest Dermatol. 1990 Aug;95(2):119-26. doi: 10.1111/1523-1747.ep12477830.
Four overlapping cDNA clones were isolated from a lambda gt11 human placenta cDNA library using purified human IgG antibody, from a patient with bullous pemphigoid. The sequence was homologous to human placenta glutathione-S-transferase-pi (GST-pi). Using the placenta clone, epidermal cDNA clones were isolated from a human keratinocyte library. Expression of GST-pi mRNA in human skin, cultured keratinocytes and fibroblasts, and disorders of squamous hyperplasia was demonstrated by Northern blotting and in situ hybridization. Human epidermal and placental cDNA clones hybridized to the same genomic DNA fragments. Hybridization of placental cDNA to interspecific somatic cell hybrids showed retention of chromosome 11, confirming the assignment of GST 3 to the long arm of chromosome 11 by molecular means. Anti-GST-pi antibody did not give a basement membrane zone pattern, although some normal and BP sera contained antibodies to GST-pi. Human skin expresses glutathione-S-transferase-pi, which belongs to an enzyme family important for detoxification and carcinogenesis.
从一名大疱性类天疱疮患者的λgt11人胎盘cDNA文库中，使用纯化的人IgG抗体分离出四个重叠的cDNA克隆。该序列与人胎盘谷胱甘肽-S-转移酶-pi（GST-pi）同源。利用胎盘克隆，从人角质形成细胞文库中分离出表皮cDNA克隆。通过Northern印迹和原位杂交证明了GST-pi mRNA在人皮肤、培养的角质形成细胞和成纤维细胞中的表达，以及鳞状增生性疾病中的表达。人表皮和胎盘cDNA克隆与相同的基因组DNA片段杂交。胎盘cDNA与种间体细胞杂种的杂交显示11号染色体的保留，通过分子手段证实了GST 3定位于11号染色体长臂。抗GST-pi抗体未呈现基底膜带模式，尽管一些正常血清和大疱性类天疱疮血清含有抗GST-pi抗体。人皮肤表达谷胱甘肽-S-转移酶-pi，它属于对解毒和致癌作用很重要的酶家族。
