Board P G, Webb G C
Proc Natl Acad Sci U S A. 1987 Apr;84(8):2377-81. doi: 10.1073/pnas.84.8.2377.
The glutathione S-transferases (GST) (glutathione transferase; EC 2.5.1.18) are a family of enzymes responsible for the metabolism of a broad range of xenobiotics and carcinogens. A cDNA clone containing the entire amino acid coding sequence of a human GST-2 subunit has been isolated using a lambda gt11 expression library. The complete nucleotide sequence and a partial restriction map are presented. The subunit is composed of 221 amino acids with a molecular weight of 25,425 before posttranslational modification. The deduced amino acid sequence is rich in lysine, which is consistent with the relatively high pI of GST-2. The human sequence shows considerable homology with the rat Ya and Yc GST sequences but little homology with the rat GSTp and Yb subunit sequences. Southern blots of restriction digests of human DNA indicate that there may be multiple GST-2 genes. In situ hybridization of the cloned cDNA to human chromosomes produces intense labeling only over band p12 on the short arm of chromosome 6 near the centromere. This indicates that the GST-2 gene(s) are located only at this site.
谷胱甘肽S-转移酶（GST）（谷胱甘肽转移酶；EC 2.5.1.18）是一类负责多种外源性物质和致癌物代谢的酶家族。利用λgt11表达文库分离出了一个包含人GST-2亚基完整氨基酸编码序列的cDNA克隆。给出了完整的核苷酸序列和部分限制性图谱。该亚基由221个氨基酸组成，翻译后修饰前分子量为25425。推导的氨基酸序列富含赖氨酸，这与GST-2相对较高的pI一致。人类序列与大鼠Ya和Yc GST序列有相当高的同源性，但与大鼠GSTp和Yb亚基序列同源性较低。人DNA限制性消化产物的Southern印迹表明可能存在多个GST-2基因。将克隆的cDNA与人类染色体进行原位杂交，仅在靠近着丝粒的6号染色体短臂上的p12带产生强烈标记。这表明GST-2基因仅位于该位点。
