Turbitt M L, Akhurst R J, White S I, MacKie R M
Department of Dermatology, Anderson College, Glasgow, Scotland.
J Invest Dermatol. 1990 Aug;95(2):229-32. doi: 10.1111/1523-1747.ep12478077.
Biopsies of involved and uninvolved skin from five patients with plaque psoriasis and normal skin from four healthy volunteers were investigated for steady-state quantities of TGF-alpha RNA and protein by in situ hybridization and immunohistochemistry. Increased levels of TGF-alpha RNA were found only in the high-level keratinocytes of involved psoriatic skin (p less than 0.001). Elevated levels of TGF-alpha protein were seen in both the high-level and basal layers of involved psoriatic skin compared to uninvolved psoriatic and normal control skin. Elevated TGF-alpha gene expression is thus implicated in the hyperproliferation of keratinocytes and possibly the altered maturation pattern seen in psoriasis.
通过原位杂交和免疫组织化学方法，对5例斑块状银屑病患者受累及未受累皮肤活检标本以及4名健康志愿者的正常皮肤进行研究，以检测转化生长因子α（TGF-α）RNA和蛋白的稳态量。仅在银屑病受累皮肤的高水平角质形成细胞中发现TGF-α RNA水平升高（p<0.001）。与银屑病未受累皮肤及正常对照皮肤相比，在银屑病受累皮肤的高水平及基底层均可见TGF-α蛋白水平升高。因此，TGF-α基因表达升高与角质形成细胞的过度增殖有关，可能也与银屑病中所见的成熟模式改变有关。
