Schmid P, Cox D, McMaster G K, Itin P
CIBA-GEIGY Ltd., Pharma Division, Basel, Switzerland.
Arch Dermatol Res. 1993;285(6):334-40. doi: 10.1007/BF00371833.
The purpose of this study was to investigate and to compare, by in situ hybridization, gene expression of IL-1 beta, IL-8, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-alpha, p53 and c-myc in lesions and in non-involved skin of patients with psoriasis. All lesional skin biopsies showed overexpression of IL-1 beta, IL-8 TGF-alpha mRNAs. IL-1 beta hybridization signals were strong in a small number of cells localized predominantly in the dermal papillae and in the suprapapillary epidermis. Overexpression of TGF-alpha was observed in all suprabasal keratinocytes, whereas strongly elevated IL-8 mRNA expression was found to be restricted to clusters of suprabasal keratinocytes. TGF-beta 3, p53 and c-myc transcripts were clearly detected in the epidermis of all biopsies, although expression levels were comparable in lesional and non-lesional skin.
本研究的目的是通过原位杂交技术，调查并比较银屑病患者皮损及非皮损皮肤中白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）、转化生长因子-β1（TGF-β1）、转化生长因子-β2（TGF-β2）、转化生长因子-β3（TGF-β3）、转化生长因子-α（TGF-α）、p53和c-myc的基因表达情况。所有皮损皮肤活检标本均显示IL-1β、IL-8、TGF-α mRNA表达上调。IL-1β杂交信号在少数主要位于真皮乳头和乳头上方表皮的细胞中较强。在所有基底上层角质形成细胞中均观察到TGF-α表达上调，而IL-8 mRNA表达显著升高仅限于基底上层角质形成细胞簇。在所有活检标本的表皮中均能清晰检测到TGF-β3、p53和c-myc转录本，尽管皮损和非皮损皮肤中的表达水平相当。
