Togawa M
Department of Orthopedic Surgery, School of Medicine, Keio University, Tokyo, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1990 May;64(5):461-71.
In order to study congenital constriction band syndrome, we performed local cauterization of the lower limb buds of embryos in fertilized white leghorn eggs between hour 0 on day 4 (day 4-0) and day 7-0 of incubation. Constriction bands, acrosyndactyly, and amputation of digits, malformations characteristic of this syndrome, were all induced by intervention after day 5-0, when digital ray formation was first observed, indicating that they all develop after ray formation. Histological examination showed that encapsulation and shedding of the lesion containing hematoma and necrotic tissue at the distal end of a digital ray led to digit amputation. When the cells encapsulating this lesion extend over another digital ray, moreover, the ray tips fuse, while the rest of the two digits involved remain discrete-resulting in acrosyndactyly.
为了研究先天性束带综合征，我们在受精的白来航鸡蛋孵化的第4天0小时（第4-0天）至第7-0天期间，对胚胎的下肢芽进行了局部烧灼。束带、并指畸形和手指截肢，这些该综合征的特征性畸形，都是在第5-0天之后进行干预诱导产生的，此时首次观察到指射线形成，这表明它们都是在射线形成之后发展而来的。组织学检查显示，指射线远端含有血肿和坏死组织的病变的包裹和脱落导致手指截肢。此外，当包裹该病变的细胞延伸到另一条指射线上时，射线尖端融合，而涉及的两个手指的其余部分保持分离，从而导致并指畸形。
