Graduate Program of Clinical Haematology Sciences, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Biol Macromol. 2013 Sep;60:393-8. doi: 10.1016/j.ijbiomac.2013.06.016. Epub 2013 Jun 24.
This study was conducted to investigate the anti-oxidant activity of the recombinant apo-c-phycocyanin (c-PC) β-subunit compared to native c-PC purified from Spirulina sp. The gene encoding the β-subunit of c-PC was successfully cloned and expressed in Escherichia coli. The anti-oxidant capacities of recombinant apo-c-PC(β) and native c-PC were evaluated by measuring their Trolox equivalent antioxidant capacities and examining their protective effects on erythrocytes from normal and homozygous haemoglobin E individuals against peroxyl radicals and hydrogen peroxide. The results demonstrated that the anti-oxidant capacities are native c-PC≫Trolox>recombinant apo-c-PC(β). Both anti-oxidant proteins can potentially protect erythrocytes from oxidative damage. Expression of c-PC in bacteria reduces the cost and time for protein production, and the recombinant protein could be further developed to obtain a more efficient protein for therapeutic purposes.
本研究旨在探讨重组脱辅基藻蓝蛋白（c-PC）β亚基与从螺旋藻中纯化的天然 c-PC 的抗氧化活性。成功地克隆了编码 c-PCβ亚基的基因，并在大肠杆菌中表达。通过测量 Trolox 当量抗氧化能力和观察它们对正常和纯合血红蛋白 E 个体的红细胞免受过氧自由基和过氧化氢的保护作用，评估了重组脱辅基藻蓝蛋白（β）和天然 c-PC 的抗氧化能力。结果表明，抗氧化能力为天然 c-PC≫Trolox>重组脱辅基藻蓝蛋白（β）。这两种抗氧化蛋白都有可能保护红细胞免受氧化损伤。在细菌中表达 c-PC 可以降低蛋白质生产的成本和时间，并且可以进一步开发重组蛋白以获得更有效的治疗蛋白。
