Department of Microbiology and Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, 1 West Beichen Road, Chaoyang District, Beijing 100101, China.
Insect Biochem Mol Biol. 2013 Sep;43(9):820-8. doi: 10.1016/j.ibmb.2013.06.006. Epub 2013 Jun 25.
Anthranilic diamides, which include the new commercial insecticide, chlorantraniliprole, are an exciting new class of chemistry that target insect ryanodine receptors. These receptors regulate release of stored intracellular calcium and play a critical role in muscle contraction. As with insects, nematodes express ryanodine receptors and are sensitive to the plant alkaloid, ryanodine. However the plant parasitic nematode, Meloidogyne incognita, is insensitive to anthranilic diamides. Expression of a full-length Drosophila melanogaster ryanodine receptor in an insect cell line confers sensitivity to the receptor agents, caffeine and ryanodine along with nanomolar sensitivity to anthranilic diamides. Replacement of a 46 amino acid segment in a highly divergent region of the Drosophila C-terminus with that from Meloidogyne results in a functional RyR which lack sensitivity to diamide insecticides. These findings indicate that this region is critical to diamide sensitivity in insect ryanodine receptors. Furthermore, this region may contribute to our understanding of the differential selectivity diamides exhibit for insect over mammalian ryanodine receptors.
邻苯二甲酰胺类化合物，包括新型商品化杀虫剂氯虫苯甲酰胺，是一类针对昆虫兰尼碱受体的新型化学物质。这些受体调节细胞内储存的钙离子释放，在肌肉收缩中起着关键作用。与昆虫一样，线虫也表达兰尼碱受体，对植物生物碱ryanodine 敏感。然而，植物寄生线虫根结线虫对邻苯二甲酰胺类化合物不敏感。在昆虫细胞系中表达全长黑腹果蝇兰尼碱受体赋予了对受体激动剂咖啡因和ryanodine 的敏感性，以及对邻苯二甲酰胺类化合物的纳摩尔敏感性。用来自根结线虫的氨基酸 46 替换果蝇 C 末端高度分化区域的一个片段，导致功能兰尼碱受体缺乏对二酰胺类杀虫剂的敏感性。这些发现表明，该区域对昆虫兰尼碱受体对二酰胺类化合物的敏感性至关重要。此外，该区域可能有助于我们理解二酰胺类化合物对昆虫兰尼碱受体的选择性差异。
