Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiao Tong University Medical Center, Xi'an, Shaanxi, China; Department of Biological Sciences, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX, USA.
Autoimmun Rev. 2013 Oct;12(12):1123-8. doi: 10.1016/j.autrev.2013.06.015. Epub 2013 Jun 24.
Since intracellular proteins involved in carcinogenesis have been shown to provoke autoantibody responses, autoantibodies can be used as probes in immunoproteomics to isolate, identify, and characterize potential tumor-associated antigens (TAAs). Once a TAA is identified, several approaches will be used to comprehensively characterize and validate the identified TAA/anti-TAA systems that are potential biomarkers in certain types of cancer. Our ultimate goal is to establish rigorous criteria for designation of an autoantibody to a TAA as a cancer biomarker, examine candidate TAAs for sensitivity and specificity of anti-TAA antibody response, and further develop customized TAA arrays that can be used to enhance anti-TAA antibody detection in cancer. This review will mainly focus on the recent advances in our studies using immunoproteomic approach to identify and characterize TAAs as biomarkers in cancer.
由于已证实与致癌相关的细胞内蛋白会引发自身抗体反应,因此自身抗体可用作免疫蛋白质组学中的探针,以分离、鉴定和特征化潜在的肿瘤相关抗原 (TAA)。一旦鉴定出 TAA,就会采用多种方法全面描述和验证鉴定出的 TAA/抗 TAA 系统,这些系统可能是某些类型癌症的潜在生物标志物。我们的最终目标是为将 TAA 的自身抗体指定为癌症生物标志物制定严格的标准,检查候选 TAA 针对抗 TAA 抗体反应的灵敏度和特异性,并进一步开发可用于增强癌症中抗 TAA 抗体检测的定制 TAA 阵列。这篇综述将主要侧重于我们使用免疫蛋白质组学方法来鉴定和特征化 TAA 作为癌症生物标志物的研究进展。