Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE-405 30 Gothenburg, Sweden.
Mar Pollut Bull. 2013 Sep 15;74(1):19-31. doi: 10.1016/j.marpolbul.2013.05.042. Epub 2013 Jun 24.
This viewpoint paper explores the potential of genomics technology to provide accurate, rapid, and cost efficient observations of the marine environment. The use of such approaches in next generation marine monitoring programs will help achieve the goals of marine legislation implemented world-wide. Genomic methods can yield faster results from monitoring, easier and more reliable taxonomic identification, as well as quicker and better assessment of the environmental status of marine waters. A summary of genomic methods that are ready or show high potential for integration into existing monitoring programs is provided (e.g. qPCR, SNP based methods, DNA barcoding, microarrays, metagenetics, metagenomics, transcriptomics). These approaches are mapped to existing indicators and descriptors and a series of case studies is presented to assess the cost and added value of these molecular techniques in comparison with traditional monitoring systems. Finally, guidelines and recommendations are suggested for how such methods can enter marine monitoring programs in a standardized manner.
本观点文件探讨了基因组学技术在提供海洋环境的准确、快速和具有成本效益的观测方面的潜力。在下一代海洋监测计划中使用此类方法将有助于实现全球实施的海洋立法目标。基因组学方法可以从监测中获得更快的结果，更容易和更可靠的分类鉴定，以及更快和更好地评估海洋水域的环境状况。提供了基因组学方法的摘要，这些方法已经准备好或显示出高度整合到现有监测计划中的潜力（例如 qPCR、基于 SNP 的方法、DNA 条形码、微阵列、宏基因组学、宏基因组学、转录组学）。这些方法被映射到现有指标和描述符上，并提出了一系列案例研究，以评估这些分子技术与传统监测系统相比的成本和附加值。最后，提出了如何以标准化的方式将这些方法纳入海洋监测计划的指南和建议。
