Department of Psychiatry, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Biomed J. 2013 May-Jun;36(3):132-6. doi: 10.4103/2319-4170.113229.
Brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) have previously been found to be reduced in the prefrontal cortex of patients with schizophrenia. In this study, we tried to investigate the protein levels of BDNF and TrkB from peripheral blood in the veins of individuals with schizophrenia and health controls.
From January 2008 to November 2010, we recruited 40 schizophrenic patients and 56 healthy controls. Serum BDNF and total TrkB protein levels were detected with enzyme-linked immunosorbent assay (ELISA) kits. Outliners of BDNF and TrkB were excluded initially. Analysis of covariance (ANCOVA) with age adjustment was used for group mean differences of different groups.
After using the ANCOVA with age adjustment, the results of this work showed that BDNF presented no significant difference (F = 0.065, p = 0.800), but the serum TrkB protein level was significantly lower in schizophrenic patients than in healthy controls (F = 8.34, p = 0.005).
Our findings showed a lower TrkB protein level in serum from schizophrenia patients compared with healthy controls, indicating that the signaling transmission of BDNF/TrkB may be affected in peripheral blood from individuals with schizophrenia.
先前发现精神分裂症患者的前额皮质中脑源性神经营养因子（BDNF）和原肌球蛋白受体激酶 B（TrkB）减少。在这项研究中，我们试图从精神分裂症患者和健康对照者的外周静脉血中检测 BDNF 和 TrkB 的蛋白水平。
2008 年 1 月至 2010 年 11 月，我们招募了 40 名精神分裂症患者和 56 名健康对照者。采用酶联免疫吸附测定（ELISA）试剂盒检测血清 BDNF 和总 TrkB 蛋白水平。首先排除 BDNF 和 TrkB 的异常值。采用协方差分析（ANCOVA）进行年龄调整，以比较不同组之间的组间均值差异。
经过年龄调整的协方差分析后，本研究结果显示 BDNF 无显著差异（F = 0.065，p = 0.800），但精神分裂症患者的血清 TrkB 蛋白水平明显低于健康对照组（F = 8.34，p = 0.005）。
我们的发现表明，与健康对照组相比，精神分裂症患者血清中的 TrkB 蛋白水平较低，这表明 BDNF/TrkB 的信号转导可能在外周血中的个体中受到影响。
