用于分离和培养心脏球的两步法方案。
Two-step protocol for isolation and culture of cardiospheres.
作者信息
Chen Lijuan, Pan Yaohua, Zhang Lan, Wang Yingjie, Weintraub Neal, Tang Yaoliang
机构信息
Division of Cardiovascular Disease, Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.
出版信息
Methods Mol Biol. 2013;1036:75-80. doi: 10.1007/978-1-62703-511-8_6.
Cardiac progenitor cells (CPC) are a unique pool of progenitor cells residing in the heart that play an important role in cardiac homeostasis and physiological cardiovascular cell turnover during acute myocardial infarction (MI). Transplanting CPC into the heart has shown promise in two recent clinical trials of cardiac repair (SCIPIO & CADUCEUS). CSCs were originally isolated directly from enzymatically digested hearts followed by cell sorting using stem cell markers. However, long exposure to enzymatic digestion can affect the integrity of stem cell markers on the cell surface and also compromise stem cell function. Here, we describe a two-step procedure in which a large number of intact cardiac progenitor cells can be purified from small amount of heart tissue.
心脏祖细胞（CPC）是存在于心脏中的一类独特的祖细胞群，在心脏稳态以及急性心肌梗死（MI）期间的生理性心血管细胞更新中发挥重要作用。在最近两项心脏修复临床试验（SCIPIO和CADUCEUS）中，将CPC移植到心脏已显示出前景。心脏干细胞最初是直接从酶消化的心脏中分离出来，然后使用干细胞标志物进行细胞分选。然而，长时间暴露于酶消化会影响细胞表面干细胞标志物的完整性，也会损害干细胞功能。在此，我们描述了一种两步法，通过该方法可以从少量心脏组织中纯化出大量完整的心脏祖细胞。
Zotero 插件