从干细胞中分离细胞外囊泡。
Isolation of Extracellular Vesicles from Stem Cells.
作者信息
Chen Zixin, Li Yongjun, Yu Hong, Shen Yan, Ju Chengwei, Ma Genshan, Liu Yutao, Kim Il-Man, Weintraub Neal L, Tang Yaoliang
机构信息
Vascular Biology Center, Department of Medicine, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, CB3303A, Augusta, GA, 30912, USA.
The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou Shi, Guangdong Sheng, China.
出版信息
Methods Mol Biol. 2017;1660:389-394. doi: 10.1007/978-1-4939-7253-1_32.
Abstract
Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC -derived EV (IPSC -EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia-reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC .
摘要
移植的诱导多能干细胞（iPSC）和胚胎干细胞（ESC）在缺血组织中表现出更高的存活率，并通过旁分泌作用促进邻近细胞的存活。最近的研究表明，干细胞可以分泌细胞外囊泡（EV），其能够在细胞间传递非编码RNA，并促进供体干细胞与受体组织之间的细胞间信号传导和通信。在急性心肌缺血再灌注（MI/R）小鼠模型中，直接移植源自iPSC的EV（iPSC-EV）在促进心肌细胞存活和预防凋亡方面非常有效。在此，我们描述了一种从培养的iPSC中纯化EV的可行方案。
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