Sarsaifi K, Rosnina Y, Ariff M O, Wahid H, Hani H, Yimer N, Vejayan J, Win Naing S, Abas M O
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Reprod Domest Anim. 2013 Dec;48(6):1006-12. doi: 10.1111/rda.12206. Epub 2013 Jun 28.
This study was conducted to evaluate the response of Bali bulls (Bos javanicus) to different semen collection methods and their effects on fresh and post-thawed semen quality. The collection methods employed were electro-ejaculation (EE), transrectal massage (RM) and RM followed by EE (RM + EE). A total of 25 untrained Bali bulls (age between 2 and 4 years old) were subjected to the different semen collection methods. Fresh semen samples from all the 25 bulls were evaluated for volume, pH, general motility, live/dead ratio and abnormality using the conventional method. For fresh and frozen samples collected by EE and RM from 10 bulls, computer-assisted semen analysis system was used for precise quantitative measurement of motility, velocity and forward progression. Accucell photometer was used to measure sperm concentration in all samples, regardless fresh and frozen. Semen samples were obtained 100% of the attempts using EE, 84% using RM and 96% using RM + EE. There were no differences among the collection methods for fresh semen quality characteristics, including motility, morphology and viability, but pH and volume were higher for EE than RM and RM + EE. Higher sperm concentration was observed in semen collected by RM than the other two methods. Different age groups (2-3 and >3-4 years old) of the bulls did not show significant differences in volume, pH, sperm concentration, percentages in motility, live/dead ratio and normal sperm morphology. The quality of semen for general and progressive motility, VAP, VSL and VCL and acrosomal integrity after thawing was higher for RM than EE. In conclusion, Bali bulls appeared to respond best to EE and the combination of RM + EE than RM, as a method of semen collection, with a shorter time of stimulation required. Differences in age of the Bali bulls did not affect the semen quality.
本研究旨在评估巴厘牛（爪哇牛）对不同采精方法的反应及其对新鲜精液和冻融后精液质量的影响。采用的采精方法有电刺激采精（EE）、直肠按摩（RM）以及直肠按摩后电刺激采精（RM + EE）。共有25头未经训练的巴厘牛（年龄在2至4岁之间）接受了不同的采精方法。使用传统方法对所有25头牛的新鲜精液样本进行体积、pH值、总活力、活/死比率和异常情况评估。对于通过EE和RM从10头牛采集的新鲜和冷冻样本，使用计算机辅助精液分析系统对活力、速度和向前运动进行精确的定量测量。无论新鲜样本还是冷冻样本，均使用Accucell光度计测量所有样本中的精子浓度。使用EE时100%的采精尝试获得了精液样本，使用RM时为84%，使用RM + EE时为96%。在新鲜精液质量特征方面，包括活力、形态和生存能力，各采精方法之间没有差异，但EE采集的精液pH值和体积高于RM和RM + EE。观察到RM采集的精液中精子浓度高于其他两种方法。不同年龄组（2至3岁和大于3至4岁）的公牛在体积、pH值、精子浓度、活力百分比、活/死比率和正常精子形态方面没有显著差异。解冻后，RM采集的精液在总活力和渐进性活力、平均路径速度（VAP）、直线运动速度（VSL）和曲线运动速度（VCL）以及顶体完整性方面的质量高于EE。总之，作为一种采精方法，巴厘牛对EE以及RM + EE组合的反应似乎比对RM更好，所需刺激时间更短。巴厘牛年龄的差异不影响精液质量。
