Fitri Wan-Nor, Wahid Haron, Rosnina Yusoff, Jesse Faez Firdaus Abdullah, Aimi-Sarah Zainal Abidin, Mohd-Azmi Mohd Lila, Azlan Che' Amat, Azrolharith Muhammad Rashid, Peter Innocent Damudu, Ali Baiee Falah Hasan
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Research Centre for Wildlife, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Vet World. 2017 Jul;10(7):779-785. doi: 10.14202/vetworld.2017.779-785. Epub 2017 Jul 14.
The objective of this research is to report parameters for breeding soundness evaluation, semen extension, and cryopreservation in .
Seven healthy stags were chosen for semen collection using an electroejaculator. The collections were performed twice in a breeding season between February and June 2016. Samples were collected between 2 and 3 weeks interval, collected twice for each animal. Semen was evaluated, extended, and cryopreserved using four different extenders; Andromed, BioXcell, Triladyl, and a modified Tris-egg yolk combined with Jack.
semen characteristics according to volume (ml), color, sperm concentration (10/ml), general motility (%), progressive motility (%), and % morphology of normal spermatozoa are 0.86±0.18 ml, thin milky to milky, 1194.2±346.1 10/ml, 82.9±2.8%, 76.1±4.8%, and 83.9±4.8%, respectively.
Semen characteristics of collected by electroejaculation is good allowing for cryopreservation and future artificial insemination work. The most suitable extender for Rusa deer semen is Andromed.
本研究的目的是报告有关[具体物种未明确]繁殖健全性评估、精液稀释和冷冻保存的参数。
选用7头健康雄鹿，使用电刺激采精器采集精液。在2016年2月至6月的繁殖季节进行了两次采集。采集间隔为2至3周，每头动物采集两次。使用四种不同的稀释液对精液进行评估、稀释和冷冻保存；Andromed、BioXcell、Triladyl以及一种改良的含杰克的Tris-蛋黄稀释液。
精液特征，按体积（毫升）、颜色、精子浓度（10⁶/毫升）、总活力（%）、前进活力（%）和正常精子形态百分比计，分别为0.86±0.18毫升、稀薄乳状至乳状、1194.2±346.1×10⁶/毫升、82.9±2.8%、76.1±4.8%和83.9±4.8%。
通过电刺激采得的[具体物种未明确]精液特征良好，可用于冷冻保存及未来的人工授精工作。对于水鹿精液而言，最合适的稀释液是Andromed。
