Brick Katherine E, Sluzevich Jason C, Cappel Mark A, DiCaudo David J, Comfere Nneka I, Wieland Carilyn N
Department of Dermatology, Mayo Clinic, Rochester, MN 55905, USA.
J Cutan Pathol. 2013 Sep;40(9):807-11. doi: 10.1111/cup.12189. Epub 2013 Jun 29.
Virtual microscopy is increasingly being used in dermatopathology educational settings.
The objective is to evaluate diagnostic accuracy and attitudes between virtual microscopy and traditional glass slide microscopy among dermatology residents.
A 48-question dermatopathology examination was administered to 35 dermatology residents at three different dermatology residency training sites during the 2011-2012 academic year with half (n = 24) of the questions using the gold standard of glass slide microscopy and half (n = 24) using whole, scanned virtual slides. Correct number of questions using glass slides and virtual slides was evaluated. Participants were surveyed regarding previous experience with digital slide imaging; quality, ease of use, and speed of slide review; and overall microscopy preferences.
Overall, diagnostic accuracy was better with glass slides than virtual slides (p = 0.01). However, no statistically significant difference was found in diagnostic accuracy of first-year trainees (p > 0.99) or trainees with exposure to virtual microscopy greater than two times per month (p = 0.27). There was no overall personal preference for glass slide vs. virtual microscopy.
Different cases and questions were used for glass slides and virtual microscopy.
Diagnostic accuracy with virtual microscopy is dependent on year of residency training and prior experience with virtual microscopy.
虚拟显微镜在皮肤病理学教育环境中的应用日益广泛。
评估皮肤科住院医师在虚拟显微镜和传统载玻片显微镜检查之间的诊断准确性和态度。
在2011 - 2012学年，对三个不同皮肤科住院医师培训地点的35名皮肤科住院医师进行了一项包含48个问题的皮肤病理学考试，其中一半问题（n = 24）采用载玻片显微镜检查的金标准，另一半问题（n = 24）采用全扫描虚拟切片。评估使用载玻片和虚拟切片回答正确的问题数量。就参与者以往数字切片成像的经验、切片检查的质量、易用性和速度以及总体显微镜偏好进行了调查。
总体而言，载玻片的诊断准确性优于虚拟切片（p = 0.01）。然而，在一年级实习生（p > 0.99）或每月接触虚拟显微镜超过两次的实习生中，未发现诊断准确性有统计学显著差异（p = 0.27）。对于载玻片和虚拟显微镜检查，总体上没有个人偏好。
载玻片和虚拟显微镜检查使用了不同的病例和问题。
虚拟显微镜检查的诊断准确性取决于住院医师培训年份和以往虚拟显微镜检查的经验。
