Musika Jidapa, Khongchatee Adison, Phinchongsakuldit Jaros
School of Science, Walailak University , Nakhon Si Thammarat , Thailand and.
Mitochondrial DNA. 2014 Aug;25(4):249-50. doi: 10.3109/19401736.2013.796519. Epub 2013 Jul 1.
The complete mitochondrial genome of cobia, Rachycentron canadum, was reanalyzed and revised. The genome is 18,008 bp in length, containing 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and a control region or displacement loop (D-loop). The gene arrangement is identical to that observed in most vertebrates. Base composition on the heavy strand is 30.14% A, 25.22% C, 15.80% G and 28.84% T. The D-loop region exhibits an A + T rich pattern, containing short tandem repeats of TATATACATGG, TATATGCACAA and TATATGCACGG. The mitochondrial genome studied differs from the previously published genome in two segments; the control region to 12S and ND5 to tRNA(Glu). The 12S sequence also differs from those published in the databases. Phylogeny analyses revealed that the differences could be due to errors in sequence assembly and/or sample misidentification of the previous studies.
军曹鱼(Rachycentron canadum)的完整线粒体基因组被重新分析和修订。该基因组长度为18,008 bp,包含13个蛋白质编码基因、2个核糖体RNA(rRNA)基因、22个转运RNA(tRNA)基因以及一个控制区或位移环(D-loop)。基因排列与大多数脊椎动物中观察到的相同。重链上的碱基组成为30.14%的A、25.22%的C、15.80%的G和28.84%的T。D-loop区呈现出富含A + T的模式,包含TATATACATGG、TATATGCACAA和TATATGCACGG的短串联重复序列。所研究的线粒体基因组在两个片段上与先前发表的基因组不同;控制区至12S以及ND5至tRNA(Glu)。12S序列也与数据库中发表的序列不同。系统发育分析表明,这些差异可能是由于先前研究中序列组装错误和/或样本错误鉴定所致。