Nougairede Antoine, Bichaud Laurence, Thiberville Simon-Djamel, Ninove Laetitia, Zandotti Christine, de Lamballerie Xavier, Brouqui Philippe, Charrel Remi N
1 UMR_D 190 "Emergence des Pathologies Virales," Aix Marseille University , IRD French Institute of Research for Development, EHESP French School of Public Health, Marseille, France .
Vector Borne Zoonotic Dis. 2013 Sep;13(9):685-8. doi: 10.1089/vbz.2013.1316. Epub 2013 Jun 29.
Toscana virus (TOSV; Bunyaviridae, Phlebovirus) is an emerging arthropod-borne virus transmitted by phlebotomine sandflies. TOSV is a frequent cause of central nervous system infection during the warm season in several countries bordering the Mediterranean Sea. Here, we report a case of TOSV aseptic meningitis diagnosed in 2012 in Marseille, France. The virus strain was recovered in cell culture from the cerebrospinal fluid. New-generation sequencing based on Ion Torrent technology was used to determine its complete genome sequence. Phylogenetic analysis based on the partial L segment revealed that this isolate belongs to the lineage B together with other French, Spanish, and Moroccan strains. Although several cases of TOSV meningitis are reported in the literature, few of them are diagnosed by RT-PCR combined with virus isolation and further sequence characterization. This case report supports that virus isolation should be attempted whenever possible because this remains the gold standard technique for diagnosis of arthropod-borne viral infections.
托斯卡纳病毒（TOSV；布尼亚病毒科，白蛉病毒属）是一种新出现的节肢动物传播病毒，由白蛉传播。在几个地中海沿岸国家，TOSV是温暖季节中枢神经系统感染的常见病因。在此，我们报告2012年在法国马赛诊断的一例TOSV无菌性脑膜炎病例。病毒株从脑脊液中通过细胞培养分离得到。使用基于Ion Torrent技术的新一代测序来确定其完整基因组序列。基于部分L片段的系统发育分析表明，该分离株与其他法国、西班牙和摩洛哥毒株同属于B系。尽管文献中报道了几例TOSV脑膜炎病例，但很少通过逆转录聚合酶链反应（RT-PCR）结合病毒分离及进一步的序列特征分析来诊断。本病例报告支持在可能的情况下应尝试进行病毒分离，因为这仍然是诊断节肢动物传播病毒感染的金标准技术。
