Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 1, 5-ka Anam-dong, Sungbuk-ku, Seoul 136-713, Republic of Korea; Institute of Life Science and Natural Resources, Korea University, Seoul 136-713, Republic of Korea.
J Plant Physiol. 2013 Nov 1;170(16):1461-5. doi: 10.1016/j.jplph.2013.05.011. Epub 2013 Jun 25.
Environmental stressors, including high salt, drought, and low or high temperatures, are often associated with significant losses in agricultural productivity. Plants have evolved a diverse array of signaling pathways to modulate their development in response to various environmental challenges. Here, we report the characterization of a member of the R2R3-MYB transcription factor family, AtMyb73. The expression of AtMyb73 was up-regulated by salt stress but not by other stresses. The maximum level of AtMyb73 expression occurred at 6h of 300mM NaCl treatment. Under salt stress, atmyb73 ko mutant plants exhibited higher survival rates compare to wild type (Col-0) plants. Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis, we determined that the accumulation of salt overly sensitive (SOS) transcripts, SOS1 and SOS3, was higher in atmyb73 ko and atmyb73 eko plants than in wild type plants in response to 300mM NaCl treatment. These results indicate that AtMyb73 is a negative regulator of SOS induction in response to salt stress in Arabidopsis thaliana.
环境胁迫因子，包括高盐、干旱、低温和高温，常与农业生产力的重大损失有关。植物已经进化出多种信号通路来调节其发育，以应对各种环境挑战。在这里，我们报告了一个 R2R3-MYB 转录因子家族成员 AtMyb73 的特征。AtMyb73 的表达受盐胁迫上调，但不受其他胁迫的影响。在 300mM NaCl 处理 6 小时时，AtMyb73 的表达水平达到最大值。在盐胁迫下，atmyb73ko 突变体植物的存活率比野生型（Col-0）植物更高。通过定量反转录聚合酶链反应（qRT-PCR）分析，我们确定在 300mM NaCl 处理下，atmyb73ko 和 atmyb73eko 植物中 SOS 转录物 SOS1 和 SOS3 的积累高于野生型植物。这些结果表明，AtMyb73 是拟南芥响应盐胁迫时 SOS 诱导的负调节剂。
