School of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518000, China.
Plant Mol Biol. 2024 Mar 21;114(2):32. doi: 10.1007/s11103-024-01431-2.
Salinity is a pivotal abiotic stress factor with far-reaching consequences on global crop growth, yield, and quality and which includes strawberries. R2R3-MYB transcription factors encompass a range of roles in plant development and responses to abiotic stress. In this study, we identified that strawberry transcription factor FaMYB63 exhibited a significant upregulation in its expression under salt stress conditions. An analysis using yeast assay demonstrated that FaMYB63 exhibited the ability to activate transcriptional activity. Compared with those in the wild-type (WT) plants, the seed germination rate, root length, contents of chlorophyll and proline, and antioxidant activities (SOD, CAT, and POD) were significantly higher in FaMYB63-overexpressing Arabidopsis plants exposed to salt stress. Conversely, the levels of malondialdehyde (MDA) were considerably lower. Additionally, the FaMYB63-overexpressed Arabidopsis plants displayed a substantially improved capacity to scavenge active oxygen. Furthermore, the activation of stress-related genes by FaMYB63 bolstered the tolerance of transgenic Arabidopsis to salt stress. It was also established that FaMYB63 binds directly to the promoter of the salt overly sensitive gene SOS1, thereby activating its expression. These findings identified FaMYB63 as a possible and important regulator of salt stress tolerance in strawberries.
盐度是一个关键的非生物胁迫因素，对全球作物生长、产量和质量都有深远的影响，包括草莓。R2R3-MYB 转录因子在植物发育和对非生物胁迫的反应中具有多种作用。在本研究中，我们发现草莓转录因子 FaMYB63 在盐胁迫条件下表达显著上调。酵母测定分析表明 FaMYB63 具有激活转录活性的能力。与野生型（WT）植物相比，在盐胁迫下，FaMYB63 过表达拟南芥植物的种子发芽率、根长、叶绿素和脯氨酸含量以及抗氧化活性（SOD、CAT 和 POD）显著升高，丙二醛（MDA）含量显著降低。此外，FaMYB63 过表达的拟南芥植物具有更强的清除活性氧的能力。此外，FaMYB63 对胁迫相关基因的激活增强了转基因拟南芥对盐胁迫的耐受性。还发现 FaMYB63 可以直接结合盐过度敏感基因 SOS1 的启动子，从而激活其表达。这些发现表明 FaMYB63 可能是草莓耐盐性的一个重要调节因子。
