Department of Cardiovascular Surgery, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey.
J Surg Res. 2013 Nov;185(1):64-9. doi: 10.1016/j.jss.2013.05.085. Epub 2013 Jun 19.
We aimed to investigate the influence of intraperitoneal ozone therapy on bacterial elimination and mediastinal inflammation in experimental Staphylococcus aureus mediastinitis.
Forty Wistar-Albino rats were randomized into five groups (eight per group) as follows: uncontaminated group, untreated contaminated group, ozone group, vancomycin group, and vancomycin + ozone group. Uncontaminated group underwent upper median sternotomy. The remaining four groups were inoculated with 0.5 mL 10(8) colony-forming units/mL methicillin-resistant Staphylococcus aureus in the mediastinal and sternal layers. Untreated contaminated group had no treatment. Rats in the vancomycin group received intramuscular vancomycin (40 mg/kg/d), and ozone was administered intraperitoneally (70 μg/mL, 1 mg/kg/d) in the ozone group for the treatment of mediastinitis. Vancomycin + ozone group rats were treated by the combination of both methods. At the end of 10 d, quantitative bacterial cultures and sternal tissue samples were obtained for determination of bacterial counts and histologic degree of inflammation.
Both the vancomycin and the ozone treatments caused significant reduction of bacterial counts in quantitative bacterial cultures. Combination of vancomycin and ozone treatments resulted in further reduction of bacterial counts in mediastinum and sternum. Histologic examination of tissue samples revealed significant reduction in severity of mediastinitis related inflammation in vancomycin and vancomycin + ozone groups compared with untreated contaminated group.
Ozone therapy as an adjunct to vancomycin leads to enhanced bacterial elimination in infected sternal and mediastinal tissues in experimental methicillin-resistant Staphylococcus aureus mediastinitis. The benefit of adjuvant ozone therapy is suggested to be related to its bactericidal effect.
我们旨在研究腹腔内臭氧治疗对金黄色葡萄球菌性纵隔炎实验模型中细菌清除和纵隔炎症的影响。
40 只 Wistar-Albino 大鼠随机分为五组（每组 8 只）：未污染组、未治疗污染组、臭氧组、万古霉素组和万古霉素+臭氧组。未污染组行中上正中开胸术。其余四组在纵隔和胸骨层接种 0.5mL 10（8）菌落形成单位/mL 耐甲氧西林金黄色葡萄球菌。未治疗污染组未进行治疗。万古霉素组大鼠肌内注射万古霉素（40mg/kg/d），臭氧组腹腔内给予臭氧（70μg/mL，1mg/kg/d）治疗纵隔炎。万古霉素+臭氧组联合应用两种方法治疗。第 10 天结束时，获取定量细菌培养物和胸骨组织样本，以确定细菌计数和组织炎症的组织学程度。
万古霉素和臭氧治疗均显著降低定量细菌培养物中的细菌计数。万古霉素和臭氧联合治疗进一步降低纵隔和胸骨中的细菌计数。组织样本的组织学检查显示，与未治疗污染组相比，万古霉素和万古霉素+臭氧组的纵隔炎相关炎症严重程度显著降低。
臭氧治疗作为万古霉素的辅助治疗可增强感染性胸骨和纵隔组织中的细菌清除。辅助臭氧治疗的益处可能与其杀菌作用有关。
