Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea.
Fish Shellfish Immunol. 2013 Sep;35(3):808-14. doi: 10.1016/j.fsi.2013.06.008. Epub 2013 Jun 27.
The objective of this study was to evaluate the effect of dietary supplementation of a Rubus coreanus ethanolic extract on immunostimulatory response in white leg shrimp Penaeus vannamei. Shrimps with an average initial weight of 0.5 ± 0.04 g were collected and acclimatized for 10 days. Four experimental diets including a control diet, a probiotic diet and 0.25 and 0.5% of R. coreanus ethanolic extract (RcEE) diets were used to feed the shrimps. After 8 weeks of culture, shrimp fed with probiotic and 0.25% RcEE diet had showed significant enhancement in the growth while shrimp fed with 0.5% RcEE diet showed significantly increased expression of immune genes and antioxidant enzymes activities. One week of challenge experiments for all the four diets fed shrimps showed decreased cumulative mortality in the 0.5% RcEE diets fed shrimps, when compared with the probiotic and 0.25% RcEE diet fed shrimp groups. The results indicates that R. coreanus ethanolic extract could be used as a herbal immunostimulant for shrimps to increase its immunity and disease resistance against the bacterial pathogen, Vibrio alginolyticus.
本研究旨在评估树莓(Rubus coreanus)乙醇提取物对凡纳滨对虾(Penaeus vannamei)免疫刺激反应的影响。选择平均初始体重为 0.5±0.04g 的虾，进行 10 天的适应期。使用四种实验饲料，包括对照饲料、益生菌饲料以及 0.25%和 0.5%的树莓乙醇提取物（RcEE）饲料，对虾进行喂养。经过 8 周的培养，与益生菌和 0.25% RcEE 饲料喂养的虾相比，食用 0.5% RcEE 饲料的虾生长显著提高，同时免疫基因和抗氧化酶活性表达显著增加。对所有四种饲料喂养的虾进行为期一周的攻毒实验，结果表明，与益生菌和 0.25% RcEE 饲料喂养的虾相比，食用 0.5% RcEE 饲料的虾的累计死亡率降低。结果表明，树莓乙醇提取物可作为虾类的一种草药免疫增强剂，提高其对细菌病原体溶藻弧菌的免疫力和抗病能力。
