Institut für Anatomie, Technische Universität Dresden, Fetscherstr. 74, 01307, Dresden, Germany.
Glycoconj J. 2013 Nov;30(8):813-8. doi: 10.1007/s10719-013-9485-7. Epub 2013 Jun 30.
N-Propanoylmannosamine is an unnatural precursor of sialic acid, which is taken up by a variety of animal cells and metabolized to N-propanoylneuraminic acid. In several studies it has been demonstrated that application of unnatural precursors of sialic acids such as N-propanoylmannosamine (ManNProp) and homologues interfere with cell differentiation and proliferation of neuronal cells or embryonic stem cells. Since the function of the immune system is known to rely on the presence of sialic acid, we applied ManNProp to human peripheral blood mononuclear cells (PBMC). When culturing those lymphocytes with ManNProp 10 % of the natural sialic acid N-acetylneuraminic acid could be replaced by the newly formed N-propanoylneuraminic acid. This procedure resulted (a) in a marked stimulation in the rate of proliferation of PBMC, (b) a 10-fold increase of IL-2 production coupled with an up-regulation of its receptor CD25 on the cell surface and (c) a concomitant expression and regulation of the transferrin receptor with cell growth. The stimulation of PBMC by ManNProp might therefore introduce a new approach of immunomodulation.
N-丙酰基甘露糖胺是一种非天然的唾液酸前体，被各种动物细胞摄取并代谢为 N-丙酰基神经氨酸。在几项研究中已经证明，应用非天然的唾液酸前体，如 N-丙酰基甘露糖胺（ManNProp）及其同系物，会干扰神经元细胞或胚胎干细胞的分化和增殖。由于免疫系统的功能已知依赖于唾液酸的存在，我们将 ManNProp 应用于人外周血单核细胞（PBMC）。当用 ManNProp 培养这些淋巴细胞时，10%的天然唾液酸 N-乙酰神经氨酸可以被新形成的 N-丙酰基神经氨酸取代。这个过程导致：(a) PBMC 的增殖速度显著增加；(b) IL-2 的产生增加了 10 倍，同时细胞表面的其受体 CD25 上调；(c) 转铁蛋白受体与细胞生长同时表达和调节。因此，ManNProp 对 PBMC 的刺激可能引入了一种新的免疫调节方法。
