Li Yi, Zhang Yuang, Tao Yiqin, Huang Xianpeng, Yu Chao, Xu Haibin, Chen Jiangjie, Xia Kaishun, Shi Kesi, Zhang Yongxiang, Wang Jingkai, Shu Jiawei, Cheng Feng, Wang Shaoke, Liang Chengzhen, Li Fangcai, Zhou Xiaopeng, Chen Qixin
Department of Orthopedics Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang, China.
Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China.
Stem Cells Int. 2023 Feb 13;2023:1655750. doi: 10.1155/2023/1655750. eCollection 2023.
Cell-based regenerative therapy utilizes the differentiation potential of stem cells to rejuvenate tissues. But the dynamic fate of stem cells is calling for precise control to optimize their therapeutic efficiency. Stem cell fate is regulated by specific conditions called "microenvironments." Among the various factors in the microenvironment, the cell-surface glycan acts as a mediator of cell-matrix and cell-cell interactions and manipulates the behavior of cells. Herein, metabolic glycoengineering (MGE) is an easy but powerful technology for remodeling the structure of glycan. By presenting unnatural glycans on the surface, MGE provides us an opportunity to reshape the microenvironment and evoke desired cellular responses. In this review, we firstly focused on the determining role of glycans on cellular activity; then, we introduced how MGE influences glycosylation and subsequently affects cell fate; at last, we outlined the application of MGE in regenerative therapy, especially in the musculoskeletal system, and the future direction of MGE is discussed.
基于细胞的再生疗法利用干细胞的分化潜能来使组织年轻化。但是干细胞的动态命运需要精确控制，以优化其治疗效果。干细胞命运受称为“微环境”的特定条件调节。在微环境的各种因素中，细胞表面聚糖充当细胞与基质以及细胞与细胞相互作用的介质，并操纵细胞行为。在此，代谢糖工程（MGE）是一种简单而强大的用于重塑聚糖结构的技术。通过在表面呈现非天然聚糖，MGE为我们提供了重塑微环境并引发所需细胞反应的机会。在这篇综述中，我们首先关注聚糖对细胞活性的决定性作用；然后，我们介绍了MGE如何影响糖基化并随后影响细胞命运；最后，我们概述了MGE在再生疗法中的应用，特别是在肌肉骨骼系统中的应用，并讨论了MGE的未来方向。
